PMID- 19838757
OWN - NLM
STAT- MEDLINE
DCOM- 20100506
LR  - 20141120
IS  - 1534-4681 (Electronic)
IS  - 1068-9265 (Linking)
VI  - 17
IP  - 2
DP  - 2010 Feb
TI  - Characterizing the HER2/neu status and metastatic potential of breast cancer 
      stem/progenitor cells.
PG  - 613-23
LID - 10.1245/s10434-009-0730-z [doi]
AB  - INTRODUCTION: Treatment resistance, long latency, and high recurrence rates 
      suggest that breast cancers arise from defective breast stem cells. HYPOTHESIS: 
      Within cancers, subpopulations of cells will demonstrate differences in 
      stem/progenitor potential, HER2/neu amplification, and gene expression. Related 
      cells will be found in normal breast tissue. METHODS: ER-/PR-/HER2/neu + breast 
      cancer cells were flow-sorted into subpopulations: (A) CD49f(+) CD24(-), (B) 
      CD49f(+)CD24(+), (C) CD49f CD24(-), and (D) CD49f(-)CD24(+). Gel matrix cell 
      invasion, fluorescence in situ hybridization (FISH) HER2/neu amplification, and 
      qRT-PCR gene expression were measured in all groups. Cells from sorted groups 
      were implanted into rat brains. Resultant tumors were analyzed by 
      immunohistochemistry (IHC) and FISH. Normal breast tissue was examined by IHC. 
      RESULTS: Tumor development varied among sorted groups (25-75%), but was highest 
      in group A. Tumor cells were mostly CD49f(-)CD24(-), with variable fractions of 
      other stem/progenitor cells. Tumors showed HER2/neu amplification, but fewer 
      chromosome 17 per cell than inoculates. Group A tumors exhibited cells with 
      normal chromosome 17 copy number and near normal HER2/neu amplification. Cell 
      invasion was 61% higher in unsorted cells and 34-42% in sorted groups compared 
      with controls. Sorted groups showed significantly different expression of 
      development, proliferation, and invasion associated genes. In normal breast 
      tissue, CD49f(+) cells were identified in CD14(+) CK19(-) basal epithelial layers 
      of mammary glands; these were 95% CD24(+) and 60% CD44(+). CONCLUSIONS: Breast 
      cancer stem/progenitor cell populations differ in tumor-initiating potential but 
      are not solely responsible for metastasis. Cancer stem/progenitor cells are less 
      polyploid than cancer cells in general and may not be HER2/neu amplified. In 
      normal breast tissue, breast stem/progenitor cell-like populations are present.
FAU - Pommier, SuEllen J
AU  - Pommier SJ
AD  - Division of Surgical Oncology, Department of General Surgery, Oregon Health & 
      Science University, Portland, OR, USA. pommiers@ohsu.edu
FAU - Quan, Glenda G
AU  - Quan GG
FAU - Christante, Dara
AU  - Christante D
FAU - Muller, Patrick
AU  - Muller P
FAU - Newell, Amy E H
AU  - Newell AE
FAU - Olson, Susan B
AU  - Olson SB
FAU - Diggs, Brian
AU  - Diggs B
FAU - Muldoon, Leslie
AU  - Muldoon L
FAU - Neuwelt, Edward
AU  - Neuwelt E
FAU - Pommier, Rodney F
AU  - Pommier RF
LA  - eng
PT  - Journal Article
DEP - 20091017
PL  - United States
TA  - Ann Surg Oncol
JT  - Annals of surgical oncology
JID - 9420840
RN  - 0 (Antigens, CD)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Blotting, Western
MH  - Brain Neoplasms/metabolism/*pathology
MH  - Breast/metabolism/*pathology
MH  - Breast Neoplasms/metabolism/*pathology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Female
MH  - Fibroblasts/cytology/metabolism
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - In Situ Hybridization, Fluorescence
MH  - Neoplasm Invasiveness
MH  - Neoplasm Metastasis
MH  - Neoplastic Stem Cells/metabolism/*pathology
MH  - Oligonucleotide Array Sequence Analysis
MH  - Phenotype
MH  - Rats
MH  - Rats, Nude
MH  - Receptor, ErbB-2/genetics/*metabolism
EDAT- 2009/10/20 06:00
MHDA- 2010/05/07 06:00
CRDT- 2009/10/20 06:00
PHST- 2009/03/02 00:00 [received]
PHST- 2009/10/20 06:00 [entrez]
PHST- 2009/10/20 06:00 [pubmed]
PHST- 2010/05/07 06:00 [medline]
AID - 10.1245/s10434-009-0730-z [doi]
PST - ppublish
SO  - Ann Surg Oncol. 2010 Feb;17(2):613-23. doi: 10.1245/s10434-009-0730-z. Epub 2009 
      Oct 17.