PMID- 1983965
OWN - NLM
STAT- MEDLINE
DCOM- 19910118
LR  - 20190824
IS  - 0009-2797 (Print)
IS  - 0009-2797 (Linking)
VI  - 77
IP  - 1
DP  - 1991
TI  - Metabolic denitrosation of N-nitroso-N-methylaniline: detection of 
      amine-metabolites.
PG  - 81-96
AB  - The enzymatic denitrosation of N-nitroso-N-methylaniline (NMA) was investigated 
      by measuring the resulting amine metabolites when NMA was incubated with liver 
      microsomes of PB-pretreated mice. Aniline was found to be the main amine 
      metabolite. Small amounts of the secondary amine, N-methylaniline (MA) and its 
      metabolite, p-methylaminophenol (p-MAP), could also be detected. Incubation of MA 
      resulted in the formation of aniline and p-MAP. The velocity of the metabolism of 
      MA was somewhat faster than that of NMA. On the basis of the measured Vmax values 
      the formation of aniline from MA or from NMA proceeded at nearly identical rates. 
      The dissociation constants as a measure of binding affinity to cytochrome (cyt.) 
      P-450 were determined by measuring the binding spectra. NMA has one Ks of 3.1 mM, 
      whereas MA shows two apparent Ks values, 650 microM and 25 mM, respectively. The 
      results are discussed in relation to the enzymatic mechanism of denitrosation of 
      NMA.
FAU - Scheper, T
AU  - Scheper T
AD  - Max von Pettenkopfer Institute, Federal Health Office, Berlin, F.R.G.
FAU - Appel, K E
AU  - Appel KE
FAU - Schunack, W
AU  - Schunack W
FAU - Somogyi, A
AU  - Somogyi A
FAU - Hildebrandt, A G
AU  - Hildebrandt AG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Chem Biol Interact
JT  - Chemico-biological interactions
JID - 0227276
RN  - 0 (Aniline Compounds)
RN  - 0 (Nitrosamines)
RN  - 9SM68I29WQ (N-methyl-N-nitrosoaniline)
RN  - M43H21IO8R (Dimethylnitrosamine)
RN  - SIR7XX2F1K (aniline)
RN  - YQE403BP4D (Phenobarbital)
SB  - IM
MH  - Aniline Compounds/*metabolism
MH  - Animals
MH  - Dimethylnitrosamine/metabolism
MH  - Female
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Mice
MH  - Microsomes, Liver/metabolism
MH  - Nitrosamines/*metabolism
MH  - Phenobarbital/pharmacology
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 0009-2797(91)90007-T [pii]
AID - 10.1016/0009-2797(91)90007-t [doi]
PST - ppublish
SO  - Chem Biol Interact. 1991;77(1):81-96. doi: 10.1016/0009-2797(91)90007-t.