PMID- 19840216
OWN - NLM
STAT- MEDLINE
DCOM- 20100208
LR  - 20100419
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Linking)
VI  - 112
IP  - 1
DP  - 2010 Jan
TI  - Hippocampal seizures alter the expression of the pannexin and connexin 
      transcriptome.
PG  - 92-102
LID - 10.1111/j.1471-4159.2009.06431.x [doi]
AB  - Some forms of seizure activity can be stopped by gap junctional (GJ) blockade. 
      Here, we found that GJ blockers attenuate hippocampal seizure activity induced by 
      a novel seizuregenic protocol using Co(2+). We hypothesized that this activity 
      may occur because of the altered expression of connexin (Cx) and/or pannexin 
      (Panx) mRNAs and protein. We found a 1.5-, 1.4-, and 2-fold increase in Panx1, 
      Panx2, and Cx43 mRNAs, respectively. Significant post-translational modifications 
      of the proteins Cx43 and Panx1 were also observed after the Co(2+) treatment. No 
      changes were observed in the presence of tetrodotoxin, indicating that seizure 
      activity is required for these alterations in expression, rather than the Co(2+) 
      treatment itself. Further analysis of the QPCR data showed that the Cx and Panx 
      transcriptome becomes remarkably re-organized. Pannexin (Panxs 1 and 2) and glial 
      connexin mRNA became highly correlated to one another; suggesting that these 
      genes formed a transcriptomic network of coordinated gene expression, perhaps 
      facilitating seizure induction. These data show that seizure activity 
      up-regulates the expression of both glial and neuronal GJ mRNAs and protein while 
      inducing a high degree of coordinate expression of the GJ transcriptome.
FAU - Mylvaganam, Shanthini
AU  - Mylvaganam S
AD  - Division of Fundamental Neurobiology, Toronto Western Research Institute, 
      University Health Network, Toronto, Ontario, Canada.
FAU - Zhang, Liang
AU  - Zhang L
FAU - Wu, Chiping
AU  - Wu C
FAU - Zhang, Zhang Jane
AU  - Zhang ZJ
FAU - Samoilova, Marina
AU  - Samoilova M
FAU - Eubanks, James
AU  - Eubanks J
FAU - Carlen, Peter L
AU  - Carlen PL
FAU - Poulter, Michael O
AU  - Poulter MO
LA  - eng
GR  - MOP-36415/Canadian Institutes of Health Research/Canada
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091013
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Connexin 43)
RN  - 0 (Connexins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Panx1 protein, mouse)
RN  - 0 (Panx2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Connexin 43/biosynthesis/genetics
MH  - Connexins/*biosynthesis/*genetics
MH  - *Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - Hippocampus/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nerve Tissue Proteins/biosynthesis/genetics
MH  - Seizures/*genetics/*metabolism
EDAT- 2009/10/21 06:00
MHDA- 2010/02/09 06:00
CRDT- 2009/10/21 06:00
PHST- 2009/10/21 06:00 [entrez]
PHST- 2009/10/21 06:00 [pubmed]
PHST- 2010/02/09 06:00 [medline]
AID - JNC6431 [pii]
AID - 10.1111/j.1471-4159.2009.06431.x [doi]
PST - ppublish
SO  - J Neurochem. 2010 Jan;112(1):92-102. doi: 10.1111/j.1471-4159.2009.06431.x. Epub 
      2009 Oct 13.