PMID- 19840250
OWN - NLM
STAT- MEDLINE
DCOM- 20100225
LR  - 20181201
IS  - 1478-3231 (Electronic)
IS  - 1478-3223 (Linking)
VI  - 30
IP  - 1
DP  - 2010 Jan
TI  - Hepatic changes of erythropoietin gene expression in a rat model of acute-phase 
      response.
PG  - 55-64
LID - 10.1111/j.1478-3231.2009.02131.x [doi]
AB  - An acute-phase response is the systemic reaction of an organism to insult (e.g. 
      infection, trauma and burning). It represents the 'first line' of defence of the 
      body to tissue-damaging attacks. In the present work, we used a rat model of an 
      intra-muscular turpentine oil (TO) injection to analyse erythropoietin (EPO) gene 
      expression changes in the liver, one of the main target organs of acute-phase 
      cytokines. EPO began to increase in the serum of TO-treated animals 6 h after 
      injection and reached a maximum at 24 h (125+/-20 pg/ml). The detection of total 
      RNA by polymerase chain reaction analysis showed that the levels of EPO gene 
      expression in the liver were considerably increased between 2 and 12 h by up to 
      20-fold at the peak after TO administration, followed by a gradual decrease over 
      the next 48 h, although the values remained significantly higher compared with 
      the control group. In the kidney, after a sudden slight increase, the values 
      declined progressively to 3.5-fold decrease at 12 h after the injection. In the 
      liver, a parallel upregulation of the hypoxia-inducible factor-1 (HIF-1) alpha 
      gene was observed (up to 4.7-fold increase), while HIF-2 alpha gene expression 
      remained unaltered. On the other hand, the protein of both genes became 
      detectable after the injection and increased progressively over 24 h, with a 
      subsequent decline. These results suggest that EPO may be added to the increasing 
      group of positive acute-phase proteins and the liver might represent the major 
      source of the hormone under these conditions in the rat.
FAU - Ramadori, Pierluigi
AU  - Ramadori P
AD  - Division of Gastroenterology and Endocrinology, University Medical Center 
      Gottingen, Gottingen, Germany.
FAU - Sheikh, Nadeem
AU  - Sheikh N
FAU - Ahmad, Ghayyor
AU  - Ahmad G
FAU - Dudas, Jozsef
AU  - Dudas J
FAU - Ramadori, Giuliano
AU  - Ramadori G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091013
PL  - United States
TA  - Liver Int
JT  - Liver international : official journal of the International Association for the 
      Study of the Liver
JID - 101160857
RN  - 0 (Acute-Phase Proteins)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (RNA, Messenger)
RN  - 0 (Solvents)
RN  - 11096-26-7 (Erythropoietin)
RN  - 1B37H0967P (endothelial PAS domain-containing protein 1)
RN  - XJ6RUH0O4G (Turpentine)
SB  - IM
MH  - Acute-Phase Proteins/genetics/metabolism
MH  - Acute-Phase Reaction/chemically induced/*genetics/metabolism
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors/genetics/metabolism
MH  - Disease Models, Animal
MH  - Erythropoietin/blood/*genetics
MH  - Gene Expression/*physiology
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/genetics/metabolism
MH  - Kidney/drug effects
MH  - Liver/drug effects/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Solvents/toxicity
MH  - Turpentine/toxicity
MH  - Up-Regulation/drug effects/*genetics
EDAT- 2009/10/21 06:00
MHDA- 2010/02/26 06:00
CRDT- 2009/10/21 06:00
PHST- 2009/10/21 06:00 [entrez]
PHST- 2009/10/21 06:00 [pubmed]
PHST- 2010/02/26 06:00 [medline]
AID - LIV2131 [pii]
AID - 10.1111/j.1478-3231.2009.02131.x [doi]
PST - ppublish
SO  - Liver Int. 2010 Jan;30(1):55-64. doi: 10.1111/j.1478-3231.2009.02131.x. Epub 2009 
      Oct 13.