PMID- 19843381
OWN - NLM
STAT- MEDLINE
DCOM- 20100107
LR  - 20211203
IS  - 1607-8454 (Electronic)
IS  - 1024-5332 (Linking)
VI  - 14
IP  - 5
DP  - 2009 Oct
TI  - Relationship between expression of mutant type glutathione S-transferase theta-1 
      gene and reactivity of rapamycin in myelodysplastic syndrome.
PG  - 266-70
LID - 10.1179/102453309X439827 [doi]
AB  - We previously reported mRNA expression of glutathione S-transferases theta 
      (GSTT)-1, wild type (623 bp) and mutant (500 bp), in patients with 
      myelodysplastic syndrome (MDS). The deletion of 123 bp creates a sequence that is 
      homologous to the mammalian target of rapamycin (mTOR). To analyze the function 
      of mutant GSTT-1 gene, stable transformants for the mutant and wild-type GSTT-1 
      gene, respectively, were established. In this study, the expression of the wild 
      and mutant type of the GSTT-1 gene of those stable transformants in cell lines 
      and in bone marrow cells from MDS patients by reverse-transcription polymerase 
      chain reaction (RT-PCR) was observed in the presence or absence of rapamycin. 
      Significant growth inhibition by rapamycin was observed among stable 
      transformants for the mutant GSTT-1 gene, but not wild type GSTT-1 gene, and was 
      indicative of typical apoptosis.
FAU - Maeda, Yasuhiro
AU  - Maeda Y
AD  - Division of Hematology, Department of Internal Medicine, Kinki University School 
      of Medicine, Osaka, Japan. ymaeda@med.kindai.ac.jp
FAU - Yamaguchi, Terufumi
AU  - Yamaguchi T
FAU - Sasakawa, Atsushi
AU  - Sasakawa A
FAU - Tanaka, Miyako
AU  - Tanaka M
FAU - Morita, Yasuyoshi
AU  - Morita Y
FAU - Kawata, Shuhei
AU  - Kawata S
FAU - Watanabe, Katsuya
AU  - Watanabe K
FAU - Hirase, Chikara
AU  - Hirase C
FAU - Takai, Shunsuke
AU  - Takai S
FAU - Miyatake, Junichi
AU  - Miyatake J
FAU - Tatsumi, Yoichi
AU  - Tatsumi Y
FAU - Kanamaru, Akihisa
AU  - Kanamaru A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Hematology
JT  - Hematology (Amsterdam, Netherlands)
JID - 9708388
RN  - 0 (Antibiotics, Antineoplastic)
RN  - EC 2.5.1.- (glutathione S-transferase T1)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Antibiotics, Antineoplastic/*pharmacology
MH  - Apoptosis/drug effects/genetics
MH  - Gene Expression Regulation, Enzymologic/*drug effects/genetics
MH  - Glutathione Transferase/*biosynthesis/genetics
MH  - HL-60 Cells
MH  - Humans
MH  - K562 Cells
MH  - *Mutation
MH  - Myelodysplastic Syndromes/drug therapy/*enzymology/genetics
MH  - Protein Kinases/genetics/metabolism
MH  - Sirolimus/*pharmacology
MH  - TOR Serine-Threonine Kinases
EDAT- 2009/10/22 06:00
MHDA- 2010/01/08 06:00
CRDT- 2009/10/22 06:00
PHST- 2009/10/22 06:00 [entrez]
PHST- 2009/10/22 06:00 [pubmed]
PHST- 2010/01/08 06:00 [medline]
AID - 10.1179/102453309X439827 [doi]
PST - ppublish
SO  - Hematology. 2009 Oct;14(5):266-70. doi: 10.1179/102453309X439827.