PMID- 19843711
OWN - NLM
STAT- MEDLINE
DCOM- 20100225
LR  - 20181201
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 24
IP  - 2
DP  - 2010 Feb
TI  - TLR2 plays a role in the activation of human resident renal stem/progenitor 
      cells.
PG  - 514-25
LID - 10.1096/fj.09-136481 [doi]
AB  - In the past few years, adult renal progenitor/stem cells (ARPCs) have been 
      identified in human kidneys, and particularly in Bowman's capsule and proximal 
      tubules. They may play an important role in the kidney regenerative processes and 
      might prospectively be the ideal cell type for the treatment of both acute and 
      chronic renal injury. In this study, microarray analysis identified 6 gene 
      clusters that discriminated normal human glomerular and tubular ARPCs from renal 
      proximal tubular epithelial cells and mesenchymal stem cells. The top-scored 
      pathway in the ARPC gene expression profile contained growth factor receptors and 
      immune system-related genes, including toll-like receptor 2 (TLR2). Stimulation 
      of TLR2 by ligands that mime inflammatory mediators or damage associated 
      molecular pattern molecules induced secretion of elevated amounts of monocyte 
      chemoattractant protein-1 (MCP-1), IL-6, IL-8, and C3 via NF-kappaB activation. 
      TLR2 stimulation also increased the ARPC proliferation rate, suggesting a role 
      for TLR2 in ARPC activation via autocrine signaling. Moreover, TLR2 stimulation 
      improved ARPC differentiation into renal epithelial cells and was responsible of 
      ARPC branching morphogenesis and tubule-like structures formation. For the first 
      time, this study provides a genomic characterization of renal multipotent 
      progenitor cells and shows that TLR2 found on ARPCs might be responsible for 
      their activation in the kidney, orchestrating the activation of crucial signaling 
      networks necessary for renal repair.
FAU - Sallustio, Fabio
AU  - Sallustio F
AD  - Nephrology Dialysis and Transplantation Unit, Department of Emergency and Organ 
      Transplantation, University of Bari, Policlinico, Piazza G. Cesare No 11, 70124, 
      Bari, Italy.
FAU - De Benedictis, Luc
AU  - De Benedictis L
FAU - Castellano, Giuseppe
AU  - Castellano G
FAU - Zaza, Gianluigi
AU  - Zaza G
FAU - Loverre, Antonia
AU  - Loverre A
FAU - Costantino, Vincenzo
AU  - Costantino V
FAU - Grandaliano, Giuseppe
AU  - Grandaliano G
FAU - Schena, Francesco P
AU  - Schena FP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091020
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (AC133 Antigen)
RN  - 0 (Antigens, CD)
RN  - 0 (CCL2 protein, human)
RN  - 0 (CD24 Antigen)
RN  - 0 (CD24 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Complement C3)
RN  - 0 (Glycoproteins)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (NF-kappa B)
RN  - 0 (Peptides)
RN  - 0 (TLR2 protein, human)
RN  - 0 (Toll-Like Receptor 2)
SB  - IM
MH  - AC133 Antigen
MH  - Adult
MH  - Antigens, CD/metabolism
MH  - CD24 Antigen/metabolism
MH  - Cell Culture Techniques
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Chemokine CCL2/metabolism
MH  - Clone Cells/physiology
MH  - Complement C3/metabolism
MH  - Gene Expression Profiling
MH  - Glycoproteins/metabolism
MH  - Humans
MH  - Interleukin-6/metabolism
MH  - Interleukin-8/metabolism
MH  - Kidney Glomerulus/physiology
MH  - Kidney Tubules, Proximal/*cytology/physiology
MH  - NF-kappa B/metabolism
MH  - Peptides/metabolism
MH  - Stem Cells/*physiology
MH  - Toll-Like Receptor 2/agonists/*physiology
EDAT- 2009/10/22 06:00
MHDA- 2010/02/26 06:00
CRDT- 2009/10/22 06:00
PHST- 2009/10/22 06:00 [entrez]
PHST- 2009/10/22 06:00 [pubmed]
PHST- 2010/02/26 06:00 [medline]
AID - fj.09-136481 [pii]
AID - 10.1096/fj.09-136481 [doi]
PST - ppublish
SO  - FASEB J. 2010 Feb;24(2):514-25. doi: 10.1096/fj.09-136481. Epub 2009 Oct 20.