PMID- 19844964
OWN - NLM
STAT- MEDLINE
DCOM- 20100106
LR  - 20191210
IS  - 1097-0231 (Electronic)
IS  - 0951-4198 (Linking)
VI  - 23
IP  - 22
DP  - 2009 Nov
TI  - Rapid drug detection in oral samples by porous silicon assisted laser 
      desorption/ionization mass spectrometry.
PG  - 3543-8
LID - 10.1002/rcm.4284 [doi]
AB  - The demand for analysis of oral fluid for illicit drugs has arisen with the 
      increased adoption of roadside testing, particularly in countries where changes 
      in legislation allow random roadside testing of drivers for the presence of a 
      palette of illicit drugs such as methamphetamine (MA), 
      3,4-methylenedioxymethamphetamine (MDMA) and Delta9-tetrahydrocannabinol (THC). 
      Oral samples are currently tested for such drugs at the roadside using an 
      immunoassay-based commercial test kit. Positive roadside tests are sent for 
      confirmatory laboratory analysis, traditionally by means of gas 
      chromatography/mass spectrometry (GC/MS). We present here an alternative rapid 
      analysis technique, porous silicon assisted laser desorption/ionization 
      time-of-flight mass spectrometry (pSi LDI-MS), for the high-throughput analysis 
      of oral fluids. This technique alleviates the need for sample derivatization, 
      requires only sub-microliter sample volumes and allows fast analysis (of the 
      order of seconds). In this study, the application of the technique is 
      demonstrated with real samples from actual roadside testing. The analysis of oral 
      samples resulted in detection of MA and MDMA with no extraction and analysis of 
      THC after ethyl acetate extraction. We propose that, subject to miniaturization 
      of a suitable mass spectrometer, this technique is well suited to underpin the 
      deployment of oral fluid testing in the clinic, workplace and on the roadside.
CI  - Copyright 2009 John Wiley & Sons, Ltd.
FAU - Lowe, Rachel D
AU  - Lowe RD
AD  - School of Chemistry, Physics and Earth Sciences, Flinders University, Bedford 
      Park, SA 5042, Australia.
FAU - Guild, Georgia E
AU  - Guild GE
FAU - Harpas, Peter
AU  - Harpas P
FAU - Kirkbride, Paul
AU  - Kirkbride P
FAU - Hoffmann, Peter
AU  - Hoffmann P
FAU - Voelcker, Nicolas H
AU  - Voelcker NH
FAU - Kobus, Hilton
AU  - Kobus H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Illicit Drugs)
RN  - Z4152N8IUI (Silicon)
SB  - IM
MH  - Humans
MH  - Illicit Drugs/*analysis
MH  - Porosity
MH  - Saliva/*chemistry
MH  - Silicon/chemistry
MH  - Spectrometry, Mass, Electrospray Ionization/instrumentation/*methods
MH  - Substance Abuse Detection/*methods
EDAT- 2009/10/22 06:00
MHDA- 2010/01/07 06:00
CRDT- 2009/10/22 06:00
PHST- 2009/10/22 06:00 [entrez]
PHST- 2009/10/22 06:00 [pubmed]
PHST- 2010/01/07 06:00 [medline]
AID - 10.1002/rcm.4284 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2009 Nov;23(22):3543-8. doi: 10.1002/rcm.4284.