PMID- 19845536
OWN - NLM
STAT- MEDLINE
DCOM- 20091202
LR  - 20211020
IS  - 1600-0463 (Electronic)
IS  - 0903-4641 (Print)
IS  - 0903-4641 (Linking)
VI  - 117
IP  - 11
DP  - 2009 Nov
TI  - Immune hierarchy among HIV-1 CD8+ T cell epitopes delivered by dendritic cells 
      depends on MHC-I binding irrespective of mode of loading and immunization in 
      HLA-A*0201 mice.
PG  - 849-55
LID - 10.1111/j.1600-0463.2009.02544.x [doi]
AB  - Recent human immunodeficiency virus type 1 (HIV-1) vaccination strategies aim at 
      targeting a broad range of cytotoxic T lymphocyte (CTL) epitopes from different 
      HIV-1 proteins by immunization with multiple CTL epitopes simultaneously. 
      However, this may establish an immune hierarchical response, where the immune 
      system responds to only a small number of the epitopes administered. To evaluate 
      the feasibility of such vaccine strategies, we used the human leukocyte antigen 
      (HLA)-A*0201 transgenic (tg) HHD murine in vivo model and immunized with 
      dendritic cells pulsed with seven HIV-1-derived HLA-A*0201 binding CTL epitopes. 
      The seven peptides were simultaneously presented on the same dendritic cell (DC) 
      or on separate DCs before immunization to one or different lymphoid compartments. 
      Data from this study showed that the T-cell response, as measured by cytolytic 
      activity and gamma-interferon (IFN-gamma)-producing CD8(+) T cells, mainly 
      focused on two of seven administered epitopes. The magnitude of individual T-cell 
      responses induced by immunization with multiple peptides correlated with their 
      individual immunogenicity that depended on major histocompatibility class I 
      binding and was not influenced by mode of loading or mode of immunization. These 
      findings may have implications for the design of vaccines based on DCs when using 
      multiple epitopes simultaneously.
FAU - Kloverpris, Henrik N
AU  - Kloverpris HN
AD  - Department of Virology, Statens Serum Institut, Copenhagen, Denmark.
FAU - Karlsson, Ingrid
AU  - Karlsson I
FAU - Thorn, Mette
AU  - Thorn M
FAU - Buus, Soren
AU  - Buus S
FAU - Fomsgaard, Anders
AU  - Fomsgaard A
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 0 (AIDS Vaccines)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A*02:01 antigen)
RN  - 0 (HLA-A2 Antigen)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - AIDS Vaccines/*immunology/standards
MH  - Animals
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Dendritic Cells/immunology/virology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitopes, T-Lymphocyte/*immunology
MH  - Flow Cytometry
MH  - HIV Infections/*immunology/prevention & control
MH  - HIV-1/*immunology
MH  - HLA-A Antigens/*immunology
MH  - HLA-A2 Antigen
MH  - Interferon-gamma/immunology
MH  - Mice
MH  - Mice, Transgenic
MH  - T-Lymphocytes, Cytotoxic/immunology/virology
PMC - PMC2774155
EDAT- 2009/10/23 06:00
MHDA- 2009/12/16 06:00
CRDT- 2009/10/23 06:00
PHST- 2009/10/23 06:00 [entrez]
PHST- 2009/10/23 06:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - APM2544 [pii]
AID - 10.1111/j.1600-0463.2009.02544.x [doi]
PST - ppublish
SO  - APMIS. 2009 Nov;117(11):849-55. doi: 10.1111/j.1600-0463.2009.02544.x.