PMID- 19845911
OWN - NLM
STAT- MEDLINE
DCOM- 20100517
LR  - 20231213
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 74
IP  - 6
DP  - 2009 Dec
TI  - A high-throughput Taqman approach for the discrimination of HLA-E alleles.
PG  - 514-9
LID - 10.1111/j.1399-0039.2009.01375.x [doi]
AB  - An association between human leukocyte antigen (HLA) molecules and the incidence 
      of various diseases has been recognized for years. Molecules of the HLA system 
      also exert an important role on the clinical outcome after transplantation such 
      as stem cell transplantation. The conventional HLA typing method is sequence 
      based typing (SBT) which is reliable but laborious. The goal of this study was to 
      develop a high-throughput Taqman assay to screen large panels for HLA-E alleles. 
      Two functional HLA-E alleles, *0101 and *0103, have been identified. We set up 
      the Taqman assay with genomic DNA as template to discriminate the three HLA-E 
      genotypes: homozygous HLA-E*0101, heterozygous *0101, *0103 and homozygous *0103. 
      This Taqman approach was validated by the comparison of results obtained with the 
      typing results acquired by sequenced base typing (SBT). Additional screening of a 
      large panel showed the pronounced discriminative capacity of the Taqman assay for 
      HLA-E allele typing. The Taqman assay is a fast, reliable and consistent HLA-E 
      allele typing method, especially useful to screen large panels.
FAU - Paquay, M M
AU  - Paquay MM
AD  - Department of Medical Immunology, University Medical Centre Utrecht (UMCU), 
      Utrecht, The Netherlands.
FAU - Schellekens, J
AU  - Schellekens J
FAU - Tilanus, M G J
AU  - Tilanus MG
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091021
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - *Alleles
MH  - Base Sequence
MH  - HLA Antigens/*genetics
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Molecular Sequence Data
MH  - HLA-E Antigens
EDAT- 2009/10/23 06:00
MHDA- 2010/05/18 06:00
CRDT- 2009/10/23 06:00
PHST- 2009/10/23 06:00 [entrez]
PHST- 2009/10/23 06:00 [pubmed]
PHST- 2010/05/18 06:00 [medline]
AID - TAN1375 [pii]
AID - 10.1111/j.1399-0039.2009.01375.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2009 Dec;74(6):514-9. doi: 10.1111/j.1399-0039.2009.01375.x. 
      Epub 2009 Oct 21.