PMID- 19846508
OWN - NLM
STAT- MEDLINE
DCOM- 20100121
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 84
IP  - 1
DP  - 2010 Jan
TI  - 17-beta estradiol promotion of herpes simplex virus type 1 reactivation is 
      estrogen receptor dependent.
PG  - 565-72
LID - 10.1128/JVI.01374-09 [doi]
AB  - Correlations between estrogen and herpes simplex virus (HSV) reactivation from 
      latency have been suggested by numerous clinical reports, but causal associations 
      are not well delineated. In a murine HSV-1 corneal infection model, we establish 
      17-beta estradiol (17-betaE) treatment of latently infected ovariectomized mice 
      induces viral reactivation, as demonstrated by increased viral load and increased 
      immediate-early viral gene expression in the latently infected trigeminal ganglia 
      (TG). Interestingly, the increased HSV reactivation occurred in the absence of 
      inhibition of viral specific CD8(+) T-cell effector function. 17-betaE 
      administration increased HSV reactivation in CD45(+) cell-depleted TG explant 
      cultures, providing further support that leukocyte-independent effects on 
      latently infected neurons were responsible for the increased reactivation. The 
      drug-induced increases in HSV copy number were not recapitulated upon in vivo 
      treatment of latently infected estrogen receptor alpha-deficient mice, evidence 
      that HSV reactivation promoted by 17-betaE was estrogen receptor dependent. These 
      findings provide additional framework for the emerging conceptualization of HSV 
      latency as a dynamic process maintained by complex interactions among multiple 
      cooperative and competing host, viral, and environmental forces. Additional 
      research is needed to confirm whether pregnancy or hormonal contraceptives 
      containing 17-betaE also promote HSV reactivation from latency in an estrogen 
      receptor-dependent manner.
FAU - Vicetti Miguel, Rodolfo D
AU  - Vicetti Miguel RD
AD  - Graduate Program in Immunology, Department of Ophthalmology, University of 
      Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA. [corrected].
FAU - Sheridan, Brian S
AU  - Sheridan BS
FAU - Harvey, Stephen A K
AU  - Harvey SA
FAU - Schreiner, Robert S
AU  - Schreiner RS
FAU - Hendricks, Robert L
AU  - Hendricks RL
FAU - Cherpes, Thomas L
AU  - Cherpes TL
LA  - eng
GR  - T32 AI060525/AI/NIAID NIH HHS/United States
GR  - P30EY08098/EY/NEI NIH HHS/United States
GR  - R01 EY005945/EY/NEI NIH HHS/United States
GR  - T32 AI 060525/AI/NIAID NIH HHS/United States
GR  - P30 EY008098/EY/NEI NIH HHS/United States
GR  - K23 AI064396/AI/NIAID NIH HHS/United States
GR  - R01 EY05945/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Receptors, Estrogen)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
EIN - J Virol. 2012 Aug;86(16):8917
MH  - Animals
MH  - Corneal Diseases/virology
MH  - *Estradiol
MH  - Estrogen Receptor alpha/deficiency/*physiology
MH  - Eye Infections, Viral
MH  - Female
MH  - Genes, Immediate-Early
MH  - Herpesvirus 1, Human/*physiology
MH  - Mice
MH  - Mice, Knockout
MH  - *Receptors, Estrogen
MH  - Viral Load
MH  - *Virus Activation
MH  - Virus Latency
PMC - PMC2798450
EDAT- 2009/10/23 06:00
MHDA- 2010/01/22 06:00
PMCR- 2010/07/01
CRDT- 2009/10/23 06:00
PHST- 2009/10/23 06:00 [entrez]
PHST- 2009/10/23 06:00 [pubmed]
PHST- 2010/01/22 06:00 [medline]
PHST- 2010/07/01 00:00 [pmc-release]
AID - JVI.01374-09 [pii]
AID - 1374-09 [pii]
AID - 10.1128/JVI.01374-09 [doi]
PST - ppublish
SO  - J Virol. 2010 Jan;84(1):565-72. doi: 10.1128/JVI.01374-09.