PMID- 19850337
OWN - NLM
STAT- MEDLINE
DCOM- 20100222
LR  - 20211020
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 31
IP  - 5
DP  - 2010 Feb
TI  - Fatigue and human umbilical cord stem cell seeding characteristics of calcium 
      phosphate-chitosan-biodegradable fiber scaffolds.
PG  - 840-7
LID - 10.1016/j.biomaterials.2009.09.106 [doi]
AB  - Calcium phosphate cement (CPC) has in situ-setting ability and bioactivity, but 
      the brittleness and low strength limit CPC to only non-load-bearing bone repairs. 
      Human umbilical cord mesenchymal stem cells (hUCMSCs) can be harvested without an 
      invasive procedure required for the commonly studied bone marrow MSCs. However, 
      little has been reported on hUCMSC delivery via bioactive scaffolds for bone 
      tissue engineering. The objectives of this study were to develop CPC scaffolds 
      with improved resistance to fatigue and fracture, and to investigate hUCMSC 
      delivery for bone tissue engineering. In fast fracture, CPC with 15% chitosan and 
      20% polyglactin fibers (CPC-chitosan-fiber scaffold) had flexural strength of 
      26mPa, higher than 10mPa for CPC control (p<0.05). In cyclic loading, 
      CPC-chitosan-fiber specimens that survived 2x10(6) cycles had the maximum stress 
      of 10MPa, compared to 5MPa of CPC control. CPC-chitosan-fiber specimens that 
      failed after multiple cycles had a mean stress-to-failure of 9MPa, compared to 
      5.8MPa for CPC control (p<0.05). hUCMSCs showed excellent viability when seeded 
      on CPC and CPC-chitosan-fiber scaffolds. The percentage of live cells reached 
      96-99%. Cell density was about 300cells/mm(2) at day 1; it proliferated to 
      700cells/mm(2) at day 4. Wst-1 assay showed that the stronger CPC-chitosan-fiber 
      scaffold had hUCMSC viability that matched the CPC control (p>0.1). In summary, 
      this study showed that chitosan and polyglactin fibers substantially increased 
      the fatigue resistance of CPC, and that hUCMSCs had excellent proliferation and 
      viability on the scaffolds.
FAU - Zhao, Liang
AU  - Zhao L
AD  - Department of Endodontics, Prosthodontics and Operative Dentistry, University of 
      Maryland Dental School, Baltimore, 21201, USA.
FAU - Burguera, Elena F
AU  - Burguera EF
FAU - Xu, Hockin H K
AU  - Xu HH
FAU - Amin, Nikhil
AU  - Amin N
FAU - Ryou, Heon
AU  - Ryou H
FAU - Arola, Dwayne D
AU  - Arola DD
LA  - eng
GR  - R01 DE014190-07A1S1/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190/DE/NIDCR NIH HHS/United States
GR  - R01 DE14190/DE/NIDCR NIH HHS/United States
GR  - R01 DE17974/DE/NIDCR NIH HHS/United States
GR  - R01 DE017974/DE/NIDCR NIH HHS/United States
GR  - R01 DE016904/DE/NIDCR NIH HHS/United States
GR  - R01 DE16904/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190-07A1/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190-08/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20091021
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Bone Substitutes)
RN  - 0 (Calcium Phosphates)
RN  - 9012-76-4 (Chitosan)
RN  - 97Z1WI3NDX (calcium phosphate)
SB  - IM
MH  - *Absorbable Implants
MH  - Bone Substitutes/*chemistry
MH  - Calcium Phosphates/*chemistry
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chitosan/*chemistry
MH  - Compressive Strength
MH  - Fetal Blood/cytology
MH  - Guided Tissue Regeneration/*instrumentation
MH  - Hardness
MH  - Materials Testing
MH  - Mesenchymal Stem Cells/*cytology/physiology
MH  - Osteoblasts/cytology/physiology
MH  - Osteogenesis/*physiology
MH  - Tensile Strength
PMC - PMC2989688
MID - NIHMS250260
EDAT- 2009/10/24 06:00
MHDA- 2010/02/23 06:00
PMCR- 2011/02/01
CRDT- 2009/10/24 06:00
PHST- 2009/09/15 00:00 [received]
PHST- 2009/09/29 00:00 [accepted]
PHST- 2009/10/24 06:00 [entrez]
PHST- 2009/10/24 06:00 [pubmed]
PHST- 2010/02/23 06:00 [medline]
PHST- 2011/02/01 00:00 [pmc-release]
AID - S0142-9612(09)01074-6 [pii]
AID - 10.1016/j.biomaterials.2009.09.106 [doi]
PST - ppublish
SO  - Biomaterials. 2010 Feb;31(5):840-7. doi: 10.1016/j.biomaterials.2009.09.106. Epub 
      2009 Oct 21.