PMID- 19851731
OWN - NLM
STAT- MEDLINE
DCOM- 20091216
LR  - 20211020
IS  - 1976-3794 (Electronic)
IS  - 1225-8873 (Linking)
VI  - 47
IP  - 5
DP  - 2009 Oct
TI  - Development and validation of a recombinant nucleocapsid protein-based ELISA for 
      detection of the antibody to porcine reproductive and respiratory syndrome virus.
PG  - 582-8
LID - 10.1007/s12275-009-0033-x [doi]
AB  - Three indirect enzyme-linked immunosorbent assays (iELISA) based on the North 
      American like (NA-like), European like (EU-like) and co-expressed NA- and EU-like 
      recombinant nucleocapsid proteins (N-protein) of porcine reproductive and 
      respiratory syndrome virus (PRRSV) were validated for the detection of the 
      antibodies in porcine sera. A total of 422 serum samples from unvaccinated pigs 
      were tested. The cut-off value was optimized by a two-graph receiver operating 
      characteristics analysis at a 95% confidence level. This assay was validated with 
      Western blot analysis and IDEXX HerdChek ELISA. Cross-reactivity results showed 
      that iELISA was PRRSV-specific. Repeatability tests revealed that the 
      coefficients of variation of positive sera within and between runs were less than 
      10%. The results indicate that iELISA is simpler to produce and perform, 
      time-saving and suitable for large scale surveys of PRRSV infection at low cost, 
      and is potentially useful to evaluate the efficiency of various vaccines against 
      PRRSV.
FAU - Chu, Jia-Qi
AU  - Chu JQ
AD  - College of Veterinary Medicine, Chungnam National University, Daejeon, Republic 
      of Korea.
FAU - Hu, Xu-Min
AU  - Hu XM
FAU - Kim, Myung-Cheol
AU  - Kim MC
FAU - Park, Chang-Sik
AU  - Park CS
FAU - Jun, Moo-Hyung
AU  - Jun MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20091024
PL  - Korea (South)
TA  - J Microbiol
JT  - Journal of microbiology (Seoul, Korea)
JID - 9703165
RN  - 0 (Antibodies, Viral)
RN  - 0 (Nucleocapsid Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Cross Reactions
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Molecular Sequence Data
MH  - Nucleocapsid Proteins/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/isolation & 
      purification
MH  - Predictive Value of Tests
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 2009/10/24 06:00
MHDA- 2009/12/17 06:00
CRDT- 2009/10/24 06:00
PHST- 2009/02/05 00:00 [received]
PHST- 2009/05/20 00:00 [accepted]
PHST- 2009/10/24 06:00 [entrez]
PHST- 2009/10/24 06:00 [pubmed]
PHST- 2009/12/17 06:00 [medline]
AID - 10.1007/s12275-009-0033-x [doi]
PST - ppublish
SO  - J Microbiol. 2009 Oct;47(5):582-8. doi: 10.1007/s12275-009-0033-x. Epub 2009 Oct 
      24.