PMID- 19853039
OWN - NLM
STAT- MEDLINE
DCOM- 20100423
LR  - 20121115
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 28
IP  - 1
DP  - 2010 Jan
TI  - Functional immune response in Pecten maximus: combined effects of a 
      pathogen-associated molecular pattern and PAH exposure.
PG  - 249-52
LID - 10.1016/j.fsi.2009.10.018 [doi]
AB  - Pathogen-associated molecular patterns (PAMPs) enable recognition of structures 
      present in microorganisms such as lipopolysaccharides (LPS). LPS are an essential 
      constituent of the outer membrane of Gram-negative bacteria, stimulating the 
      innate immune system of invertebrates. Here, LPS from Escherichia coli (055:B5) 
      were used to investigate the functional immune response of Pecten maximus after 
      stimulation with a PAMP and to determine the combined effect of a phenanthrene 
      exposure and LPS challenge. Organisms were exposed to 200 mug l(-1) phenanthrene 
      and after 7 d were injected with either physiological saline (injection controls) 
      or LPS solution, and returned to their respective exposure tanks. Haemolymph was 
      sampled from the scallops 48 h post-injection and immune function was assessed 
      using a combination of cellular biological responses. The LPS challenge 
      significantly altered the immune response in P. maximus with increased cell 
      counts and phagocytic activity. An immunosuppressive effect of phenanthrene was 
      also observed in this study; however, exposure to phenanthrene did not 
      significantly impair the organism's ability to respond to a PAMP challenge. The 
      overall level of phagocytosis and cytotoxic capability following the LPS 
      challenge was lower in phenanthrene exposed scallops and may have consequences 
      for disease resistance in this commercially-exploited species.
CI  - Copyright 2009 Elsevier Ltd. All rights reserved.
FAU - Hannam, Marie L
AU  - Hannam ML
AD  - Ecotoxicology and Stress Biology Research Centre, School of Biomedical and 
      Biological Sciences, University of Plymouth, Drake Circus, Plymouth, Devon PL4 
      8AA, UK. marie.hannam@plymouth.ac.uk
FAU - Bamber, Shaw D
AU  - Bamber SD
FAU - Galloway, Tamara S
AU  - Galloway TS
FAU - John Moody, A
AU  - John Moody A
FAU - Jones, Malcolm B
AU  - Jones MB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091021
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Phenanthrenes)
RN  - 448J8E5BST (phenanthrene)
SB  - IM
MH  - Animals
MH  - Immunity/drug effects
MH  - Immunity, Innate/drug effects/immunology
MH  - Immunosuppressive Agents/pharmacology
MH  - Leukocyte Count
MH  - Lipopolysaccharides/immunology
MH  - Pecten/drug effects/*immunology
MH  - Phagocytosis/drug effects/immunology/physiology
MH  - Phenanthrenes/pharmacology
EDAT- 2009/10/27 06:00
MHDA- 2010/04/24 06:00
CRDT- 2009/10/27 06:00
PHST- 2009/07/13 00:00 [received]
PHST- 2009/10/14 00:00 [revised]
PHST- 2009/10/14 00:00 [accepted]
PHST- 2009/10/27 06:00 [entrez]
PHST- 2009/10/27 06:00 [pubmed]
PHST- 2010/04/24 06:00 [medline]
AID - S1050-4648(09)00339-8 [pii]
AID - 10.1016/j.fsi.2009.10.018 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2010 Jan;28(1):249-52. doi: 10.1016/j.fsi.2009.10.018. 
      Epub 2009 Oct 21.