PMID- 19853838
OWN - NLM
STAT- MEDLINE
DCOM- 20100330
LR  - 20161125
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 54
IP  - 12
DP  - 2009 Dec
TI  - Involvement of medullary dorsal horn glial cell activation in mediation of 
      masseter mechanical allodynia induced by experimental tooth movement.
PG  - 1143-50
LID - 10.1016/j.archoralbio.2009.09.006 [doi]
AB  - OBJECTIVE: To investigate the involvement of microglial and astrocytic activation 
      in the medullary dorsal horn (MDH) during the mediation of masseter area 
      allodynia induced by experimental tooth movement (ETM). DESIGN: Five groups of 
      adult Sprague-Dawley rats (n=60) were divided into control (CON), minocycline 
      (MIN), ETM, and 10mg/kg or 30mg/kg MIN plus ETM (METM) groups. The 
      upper-first-molar was moved mesially for rats in ETM and METM groups. Rats were 
      pre-injected with minocycline in the MIN (30mg/kg) and METM (10mg/kg or 30mg/kg) 
      groups. Pressure pain threshold (PPT) in masseter area was tested from day 0 to 
      14 for all 5 groups. Immunohistochemistry against OX42 (microglial marker) or 
      GFAP (astrocytic maker) in the MDH was examined at days 1, 3, 7 and 14 for CON, 
      MIN and 30mg/kg METM groups. RESULTS: Baseline PPT was expectedly seen in either 
      CON or MIN groups, masseter mechanical allodynia was detected in the ETM group 
      from day 4 to 13 (P<0.05). OX42 expression level at days 1, 3 and 7, and GFAP 
      expression level at days 3, 7 and 14 were higher in ETM (P<0.05), but not in 
      30mg/kg METM, than in CON group. Minocycline reduced activation of microglia and 
      astrocytes, and significantly attenuated the development of masseter mechanical 
      allodynia in this model. CONCLUSIONS: These results indicate that mechanical 
      allodynia in the masseter area induced by ETM can be attenuated by minocycline. 
      Activation of microglia, possibly together with subsequent activation of 
      astrocytes, seems to contribute to masseter mechanical allodynia.
FAU - Liu, Xiao-Dong
AU  - Liu XD
AD  - Department of Oral Anatomy & Physiology and TMD, The Fourth Military Medical 
      University, Xi'an, Shaanxi, PR China.
FAU - Wang, Jing-Jie
AU  - Wang JJ
FAU - Sun, Lei
AU  - Sun L
FAU - Chen, Liang-Wei
AU  - Chen LW
FAU - Rao, Zhi-Ren
AU  - Rao ZR
FAU - Duan, Li
AU  - Duan L
FAU - Cao, Rong
AU  - Cao R
FAU - Wang, Mei-Qing
AU  - Wang MQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091024
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Neuropeptide)
RN  - FYY3R43WGO (Minocycline)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology/therapeutic use
MH  - Astrocytes/drug effects/metabolism
MH  - Facial Pain/etiology/*physiopathology/prevention & control
MH  - Male
MH  - Masseter Muscle/*physiology
MH  - Microglia/drug effects/metabolism
MH  - Minocycline/pharmacology/therapeutic use
MH  - Nerve Tissue Proteins/biosynthesis
MH  - Pain Threshold
MH  - Posterior Horn Cells/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Neuropeptide/biosynthesis
MH  - *Tooth Movement Techniques/adverse effects
EDAT- 2009/10/27 06:00
MHDA- 2010/03/31 06:00
CRDT- 2009/10/27 06:00
PHST- 2009/04/24 00:00 [received]
PHST- 2009/09/18 00:00 [revised]
PHST- 2009/09/26 00:00 [accepted]
PHST- 2009/10/27 06:00 [entrez]
PHST- 2009/10/27 06:00 [pubmed]
PHST- 2010/03/31 06:00 [medline]
AID - S0003-9969(09)00245-3 [pii]
AID - 10.1016/j.archoralbio.2009.09.006 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2009 Dec;54(12):1143-50. doi: 10.1016/j.archoralbio.2009.09.006. 
      Epub 2009 Oct 24.