PMID- 19854711
OWN - NLM
STAT- MEDLINE
DCOM- 20100727
LR  - 20100622
IS  - 1468-2060 (Electronic)
IS  - 0003-4967 (Linking)
VI  - 69
IP  - 7
DP  - 2010 Jul
TI  - Blood dendritic cells in systemic lupus erythematosus exhibit altered activation 
      state and chemokine receptor function.
PG  - 1370-7
LID - 10.1136/ard.2009.111021 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) have a pivotal role in the pathogenesis of 
      systemic lupus erythematosus (SLE). Reduced numbers of blood DCs and the 
      accumulation of DCs at inflammatory sites have been observed in SLE. One crucial 
      feature of DCs is their ability to migrate. OBJECTIVE: To analyse the 
      maturation/activation state and the migratory capacity of different DC precursor 
      subsets in SLE to further elucidate their role in autoimmunity. METHODS: 
      Plasmacytoid DCs (pDCs), myeloid DCs (mDCs) and monocytes from patients with SLE, 
      healthy volunteers and healthy volunteers immunised with tetanus/diphtheria were 
      examined by flow cytometry for expression of subset-specific antigens (BDCA-2, 
      CD11c, CD14, HLA-DR), activation/maturation markers (CD83, CD86, CD40, BLyS) and 
      chemokine receptors (CCR1, CCR5, CCR7, ChemR23). Additionally, migratory capacity 
      to chemokine receptors was investigated in vitro using the chemokines RANTES, 
      CCL19 and chemerin. RESULTS: SLE monocytes and mDCs had higher CD86 and 
      B-lymphocyte stimulatory factor (BLyS) expression levels. ChemR23 expression was 
      lower in SLE pDCs and mDCs. Basal and CCL19-specific migration levels were higher 
      in SLE pDCs. Altered DC function in SLE had no correlative changes in chemokine 
      receptor expression, whereas immunisation-induced blood DC migration patterns in 
      healthy donors were accompanied by changes in chemokine receptor expression. 
      CONCLUSIONS: The phenotypic and migratory disturbances observed in SLE blood DCs 
      could result in altered distribution of DCs in peripheral tissues, contributing 
      to dysregulated immune responses and autoimmunity.
FAU - Gerl, Velia
AU  - Gerl V
AD  - Department of Rheumatology and Clinical Immunology, Charite - Universitatsmedizin 
      Berlin, Chariteplatz 1, 10117 Berlin, Germany. falk.hiepe@charite.de
FAU - Lischka, Alexandra
AU  - Lischka A
FAU - Panne, Daniel
AU  - Panne D
FAU - Grossmann, Patrick
AU  - Grossmann P
FAU - Berthold, Rita
AU  - Berthold R
FAU - Hoyer, Bimba Franziska
AU  - Hoyer BF
FAU - Biesen, Robert
AU  - Biesen R
FAU - Bruns, Anne
AU  - Bruns A
FAU - Alexander, Tobias
AU  - Alexander T
FAU - Jacobi, Annett
AU  - Jacobi A
FAU - Dorner, Thomas
AU  - Dorner T
FAU - Burmester, Gerd-Rudiger
AU  - Burmester GR
FAU - Radbruch, Andreas
AU  - Radbruch A
FAU - Hiepe, Falk
AU  - Hiepe F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091022
PL  - England
TA  - Ann Rheum Dis
JT  - Annals of the rheumatic diseases
JID - 0372355
RN  - 0 (Diphtheria Toxin)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Tetanus Toxin)
SB  - IM
MH  - Adult
MH  - Autoimmunity/immunology
MH  - Cell Differentiation/immunology
MH  - Chemotaxis/immunology
MH  - Dendritic Cells/*immunology
MH  - Diphtheria Toxin/immunology
MH  - Female
MH  - Flow Cytometry/methods
MH  - Humans
MH  - Immunophenotyping
MH  - Lupus Erythematosus, Systemic/*immunology
MH  - Middle Aged
MH  - Monocytes/immunology
MH  - Receptors, Chemokine/*blood
MH  - Tetanus Toxin/immunology
MH  - Young Adult
EDAT- 2009/10/27 06:00
MHDA- 2010/07/28 06:00
CRDT- 2009/10/27 06:00
PHST- 2009/10/27 06:00 [entrez]
PHST- 2009/10/27 06:00 [pubmed]
PHST- 2010/07/28 06:00 [medline]
AID - ard.2009.111021 [pii]
AID - 10.1136/ard.2009.111021 [doi]
PST - ppublish
SO  - Ann Rheum Dis. 2010 Jul;69(7):1370-7. doi: 10.1136/ard.2009.111021. Epub 2009 Oct 
      22.