PMID- 1986144
OWN - NLM
STAT- MEDLINE
DCOM- 19910214
LR  - 20190820
IS  - 0022-4790 (Print)
IS  - 0022-4790 (Linking)
VI  - 46
IP  - 1
DP  - 1991 Jan
TI  - Two prostate carcinoma cell lines demonstrate abnormalities in tumor suppressor 
      genes.
PG  - 31-6
AB  - Two prostate carcinoma cell lines, DU-145 and PC-3, were examined for 
      abnormalities in the retinoblastoma (Rb) and the p53 putative tumor suppressor 
      genes. We found an abnormal Rb gene product in DU-145 using Western blot 
      analysis. Polymerase chain reaction amplification followed by direct DNA 
      sequencing demonstrated a base substitution mutation that generates a stop codon 
      in exon 21. On Northern, Southern, and Western blot analysis, the p53 gene and 
      its product appear to be normal in DU-145. PC-3, however, failed to demonstrate 
      expression of either the p53 transcript on Northern blot analysis or the p53 
      protein on Western blot analysis, while the Rb gene products appeared to be 
      normal on both Northern and Western blot analysis. This work extends the 
      correlation between abnormal expression of putative tumor suppressor genes and 
      human malignancies.
FAU - Rubin, S J
AU  - Rubin SJ
AD  - Department of Radiation and Cellular Oncology, Michael Reese/University of 
      Chicago Center for Radiation Therapy, Chicago, Illinois.
FAU - Hallahan, D E
AU  - Hallahan DE
FAU - Ashman, C R
AU  - Ashman CR
FAU - Brachman, D G
AU  - Brachman DG
FAU - Beckett, M A
AU  - Beckett MA
FAU - Virudachalam, S
AU  - Virudachalam S
FAU - Yandell, D W
AU  - Yandell DW
FAU - Weichselbaum, R R
AU  - Weichselbaum RR
LA  - eng
GR  - CA 41068/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Surg Oncol
JT  - Journal of surgical oncology
JID - 0222643
RN  - 0 (DNA, Neoplasm)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
SB  - IM
MH  - Base Sequence
MH  - Chromatography, Paper
MH  - DNA, Neoplasm/analysis
MH  - Genes, Retinoblastoma
MH  - Genes, Tumor Suppressor/*genetics
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Mutation/genetics
MH  - Polymerase Chain Reaction
MH  - Prostatic Neoplasms/*genetics
MH  - RNA, Messenger/analysis
MH  - RNA, Neoplasm/analysis
MH  - Tumor Cells, Cultured
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 10.1002/jso.2930460108 [doi]
PST - ppublish
SO  - J Surg Oncol. 1991 Jan;46(1):31-6. doi: 10.1002/jso.2930460108.