PMID- 19861507
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20120823
LR  - 20211020
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Print)
IS  - 1741-427X (Linking)
VI  - 2011
DP  - 2011
TI  - Proteomic Analysis of Anti-inflammatory Effects of a Kampo (Japanese Herbal) 
      Medicine "Shoseiryuto (Xiao-Qing-Long-Tang)" on Airway Inflammation in a Mouse 
      Model.
PG  - 604196
LID - 10.1093/ecam/nep151 [doi]
LID - 604196
AB  - Effects of a Kampo (Japanese herbal) medicine "shoseiryuto (SST, 
      xiao-qing-long-tang in Chinese)", which has been used for the treatment of 
      allergic bronchial asthma clinically, were examined on ovalbumin (OVA)-sensitized 
      allergic airway inflammation model (i.e., bronchial asthma) in a mouse. When SST 
      was orally administered at 0.5 g kg(-1) day(-1) from day 1 to 6 after OVA 
      inhalation, SST reduced the inflammation in lung tissue, the number of 
      eosinophils and the OVA-specific immunoglobulin E (IgE) antibody titer in 
      bronchoalveolar lavage (BAL) fluids at 7 days after the OVA inhalation. SST also 
      reduced the airway hyperreactivity at 6 days after the OVA inhalation. Proteomic 
      analysis with the agarose two-dimensional electrophoresis showed that the 
      expression of spectrin alpha2 was reduced in the lung tissue of OVA-sensitized mice 
      and SST recovered the expression. Western blot and immunohistochemical analyses 
      of lung tissue also confirmed this result. When prednisolone was orally 
      administered at 3 mg kg(-1) day(-1) from day 1 to 6 after OVA inhalation, the 
      inflammation in lung tissue, the number of eosinophils in BAL fluids and airway 
      hyperreactivity were reduced in the OVA-sensitized mice. However, prednisolone 
      did not reduce the OVA-specific IgE antibody titer in BAL fluids and did not 
      recover the expression of spectrin alpha2 in lung tissue. These results suggest that 
      at least a part of action mechanism of SST against OVA-sensitized allergic airway 
      inflammation in a mouse model is different from that of prednisolone.
FAU - Nagai, Takayuki
AU  - Nagai T
AD  - Kitasato Institute for Life Sciences and Graduate School of Infection Control 
      Sciences, Japan.
FAU - Nakao, Marino
AU  - Nakao M
FAU - Shimizu, Yuliko
AU  - Shimizu Y
FAU - Kodera, Yoshio
AU  - Kodera Y
FAU - Oh-Ishi, Masamichi
AU  - Oh-Ishi M
FAU - Maeda, Tadakazu
AU  - Maeda T
FAU - Yamada, Haruki
AU  - Yamada H
LA  - eng
PT  - Journal Article
DEP - 20110310
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC3136714
EDAT- 2009/10/29 06:00
MHDA- 2009/10/29 06:01
PMCR- 2011/03/10
CRDT- 2009/10/29 06:00
PHST- 2009/07/15 00:00 [received]
PHST- 2009/08/25 00:00 [accepted]
PHST- 2009/10/29 06:00 [entrez]
PHST- 2009/10/29 06:00 [pubmed]
PHST- 2009/10/29 06:01 [medline]
PHST- 2011/03/10 00:00 [pmc-release]
AID - nep151 [pii]
AID - 10.1093/ecam/nep151 [doi]
PST - ppublish
SO  - Evid Based Complement Alternat Med. 2011;2011:604196. doi: 10.1093/ecam/nep151. 
      Epub 2011 Mar 10.