PMID- 19861730
OWN - NLM
STAT- MEDLINE
DCOM- 20100105
LR  - 20091028
IS  - 2384-8553 (Electronic)
IS  - 0021-2571 (Linking)
VI  - 45
IP  - 3
DP  - 2009
TI  - The micronucleus assay in radiation accidents.
PG  - 260-4
AB  - The cytokinesis-block micronucleus assay in peripheral blood lymphocytes is a 
      standardised and validated technique for biodosimetry. Automated scoring of 
      micronuclei allows large scale applications as in population triage in case of 
      radiation accidents or malevolent use of radioactive sources. The dose detection 
      limit (95% confidence) of the micronucleus assay for individual dose assessment 
      is restricted to 0.2 Gy but can be decreased to 0.1 Gy by scoring centromeres in 
      micronuclei using fluorescence in situ hybridization (FISH). In the past the 
      micronucleus assay was applied for a number of large scale biomonitoring studies 
      of nuclear power plant workers and hospital workers. Baseline micronucleus 
      frequencies depend strongly on age and gender. The assay was also already used 
      for biodosimetry of radiation accidents. In a multiple endpoint biodosimetry 
      study for dose assessment of a worker exposed accidentally in 2003 to X-rays, a 
      good agreement was obtained between dose estimates resulting from the 
      micronucleus assay, the scoring of dicentrics and translocations. Automated 
      scoring of micronuclei in combination with centromere signals, allowing 
      systematic biodosimetry of exposed populations, remains a challenge for the 
      future.
FAU - Thierens, Hubert
AU  - Thierens H
AD  - Department of Basic Medical Sciences, Faculty of Medicine, University of Ghent, 
      Belgium. hubert.thierens@ugent.be
FAU - Vral, Anne
AU  - Vral A
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Italy
TA  - Ann Ist Super Sanita
JT  - Annali dell'Istituto superiore di sanita
JID - 7502520
SB  - IM
MH  - Automation
MH  - Centromere/radiation effects/ultrastructure
MH  - Humans
MH  - Lymphocytes/radiation effects
MH  - Micronucleus Tests/*methods
MH  - *Radioactive Hazard Release
MH  - Radiometry/*methods
RF  - 43
EDAT- 2009/10/29 06:00
MHDA- 2010/01/06 06:00
CRDT- 2009/10/29 06:00
PHST- 2009/10/29 06:00 [entrez]
PHST- 2009/10/29 06:00 [pubmed]
PHST- 2010/01/06 06:00 [medline]
PST - ppublish
SO  - Ann Ist Super Sanita. 2009;45(3):260-4.