PMID- 19861898 OWN - NLM STAT- MEDLINE DCOM- 20100126 LR - 20220331 IS - 1533-4066 (Electronic) IS - 1052-9551 (Linking) VI - 18 IP - 4 DP - 2009 Dec TI - Molecular diagnosis of Ewing sarcoma family of tumors: a comparative analysis of 560 cases with FISH and RT-PCR. PG - 189-99 LID - 10.1097/PDM.0b013e3181a06f66 [doi] AB - AIMS: To compare the sensitivity and specificity of fluorescence in situ hybridization (FISH) with reverse transcription polymerase chain reaction (RT-PCR) in the diagnosis of Ewing sarcoma family of tumors (ESFTs) and other small round-cell tumors (SRCTs) in formalin-fixed paraffin-embedded tissue assembled in tissue microarrays (TMAs). The second objective is to confirm the value of molecular methods and immunohistochemical (IHC) assays, to perform a differential diagnosis between ESFTs and SRCTs with similar or overlapping morphology. MATERIALS AND METHODS: A total of 560 cases were selected for the present study out the 806 cases collected from the PROgnosis and THerapeutic Targets in the Ewing's Family of TumorS project. Case selection bias included only the cases with enough material to enable the TMA construction, as FISH analysis and the majority of IHC studies were performed in TMAs. Histopathologic, IHC, and molecular assays were carried out. RESULTS: Of the 560 total cases, 411 (73.4%) were considered informative (with results by FISH and/or RT-PCR assays). From the informative cases, 382 (92.9%) were diagnosed as ESFT, 23 cases (5.6%) as non-ESFT but with specific diagnosis for another established entity, and 6 cases (1.5%) as small round cell tumors not otherwise specified. Sensitivity and specificity for the FISH assays was 96.3% and 95.2%, respectively, whereas RT-PCR presented a sensitivity of 97.5% and specificity of 92.9%. In concordant cases, both methods showed a sensitivity and specificity of 99.2% and 100%, respectively. Twenty-nine cases (7.1%) initially interpreted at morphologic level as atypical ESFTs were finally reclassified, with the support of molecular methods and IHC, as either non-ESFT with another specific histologic type or as small round cell tumors not otherwise specified. CONCLUSIONS: FISH and RT-PCR are ancillary techniques possessing high sensitivity in the diagnosis of ESFT; nevertheless, FISH is more specific than RT-PCR in the diagnosis of formalin-fixed paraffin-embedded tissue. Both methods in combination displayed the highest sensitivity and specificity. The combination of histopathologic, IHC, and molecular findings is the method of choice for the diagnosis of ESFT, as well as for the differential diagnosis with other SRCTs. FAU - Machado, Isidro AU - Machado I AD - Department of Pathology, University of Valencia, Valencia, Spain. FAU - Noguera, Rosa AU - Noguera R FAU - Pellin, Antonio AU - Pellin A FAU - Lopez-Guerrero, Jose A AU - Lopez-Guerrero JA FAU - Piqueras, Marta AU - Piqueras M FAU - Navarro, Samuel AU - Navarro S FAU - Llombart-Bosch, Antonio AU - Llombart-Bosch A LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Diagn Mol Pathol JT - Diagnostic molecular pathology : the American journal of surgical pathology, part B JID - 9204924 RN - 0 (Biomarkers, Tumor) RN - 0 (DNA, Neoplasm) RN - 0 (RNA, Neoplasm) SB - IM MH - Biomarkers, Tumor/analysis MH - Bone Neoplasms/chemistry/*diagnosis/genetics MH - DNA, Neoplasm/analysis MH - Humans MH - In Situ Hybridization, Fluorescence/*methods MH - *Molecular Diagnostic Techniques MH - Paraffin Embedding MH - Predictive Value of Tests MH - RNA, Neoplasm/analysis MH - Reverse Transcriptase Polymerase Chain Reaction/*methods MH - Sarcoma, Ewing/chemistry/*diagnosis/genetics MH - Tissue Array Analysis MH - Translocation, Genetic EDAT- 2009/10/29 06:00 MHDA- 2010/01/27 06:00 CRDT- 2009/10/29 06:00 PHST- 2009/10/29 06:00 [entrez] PHST- 2009/10/29 06:00 [pubmed] PHST- 2010/01/27 06:00 [medline] AID - 10.1097/PDM.0b013e3181a06f66 [doi] PST - ppublish SO - Diagn Mol Pathol. 2009 Dec;18(4):189-99. doi: 10.1097/PDM.0b013e3181a06f66.