PMID- 19862670
OWN - NLM
STAT- MEDLINE
DCOM- 20100604
LR  - 20211203
IS  - 1439-0221 (Electronic)
IS  - 0032-0943 (Linking)
VI  - 76
IP  - 5
DP  - 2010 Mar
TI  - Standardization and in vivo antioxidant activity of ethanol extracts of fruit and 
      leaf of Piper sarmentosum.
PG  - 418-25
LID - 10.1055/s-0029-1186279 [doi]
AB  - The present study aimed to investigate standardized ethanol extracts of fruit and 
      leaves of Piper sarmentosum for their in vivo antioxidant activity in rats using 
      a CCl (4)-induced oxidative stress model. The standardization was based on the 
      quantification of the markers pellitorine, sarmentine and sarmentosine by high 
      performance liquid chromatography (HPLC), and determination of total primary and 
      secondary metabolites. The rats, divided into 7 groups each (n = 6), were used as 
      follows: group 1 (CCl (4), negative control), group 2 (untreated, control), 
      groups 3 and 4 (fruit extract 250 and 500 mg/kg, respectively), groups 5 and 6 
      (leaf extract 250 and 500 mg/kg, respectively) and group 7 (vitamin-E 100 mg/kg, 
      positive control). The doses were administered orally for 14 days; 4 h following 
      the last dose, a single dose of CCl (4) (1.5 mg/kg) was given orally to all the 
      groups except group 2, and after 24 h, blood and liver of each animal were 
      obtained. Analysis of plasma and liver homogenate exhibited significant 
      preservation of markers of antioxidant activity, total plasma antioxidant 
      activity (TPAA), total protein (TP), superoxide dismutase (SOD), catalase (CAT), 
      and thiobarbituric acid reactive species (TBARS), in the pretreated groups as 
      compared to the CCl (4) group (p < 0.05). Histology of the liver also evidenced 
      the protection of hepatocytes against CCl (4) metabolites in the pretreated 
      groups. The results of this study indicate the IN VIVO antioxidant activity of 
      both extracts of the plant, which may be valuable to combat diseases involving 
      free radicals.
CI  - (c) Georg Thieme Verlag KG Stuttgart . New York.
FAU - Hussain, Khalid
AU  - Hussain K
AD  - School of Pharmaceutical Sciences, University Sains Malaysia, Pulau Pinang, 
      Malaysia. hussain_761@yahoo.com
FAU - Ismail, Zhari
AU  - Ismail Z
FAU - Sadikun, Amirin
AU  - Sadikun A
FAU - Ibrahim, Pazilah
AU  - Ibrahim P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091027
PL  - Germany
TA  - Planta Med
JT  - Planta medica
JID - 0066751
RN  - 0 (Allylbenzene Derivatives)
RN  - 0 (Anisoles)
RN  - 0 (Antioxidants)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Nitriles)
RN  - 0 (Plant Extracts)
RN  - 0 (Polyunsaturated Alkamides)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 0 (asarone)
RN  - 3K9958V90M (Ethanol)
RN  - 71933-54-5 (sarmentosine)
RN  - 8IS5231171 (pellitorine)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Allylbenzene Derivatives
MH  - Animals
MH  - Anisoles/isolation & purification/*standards/toxicity
MH  - Antioxidants/isolation & purification/pharmacology/*standards
MH  - Carbon Tetrachloride
MH  - Catalase/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Ethanol/chemistry
MH  - Fatty Acids, Unsaturated/isolation & purification/pharmacology/standards
MH  - Fruit/chemistry
MH  - Glucose/analogs & derivatives/isolation & purification/pharmacology/standards
MH  - Liver/drug effects/enzymology/metabolism
MH  - Male
MH  - Mouth/drug effects
MH  - Nitriles/isolation & purification/pharmacology/standards
MH  - Piper/*chemistry
MH  - Plant Extracts/isolation & purification/*standards/toxicity
MH  - Plant Leaves/chemistry
MH  - Polyunsaturated Alkamides/isolation & purification/pharmacology/standards
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Superoxide Dismutase/metabolism
MH  - Thiobarbituric Acid Reactive Substances/metabolism
EDAT- 2009/10/29 06:00
MHDA- 2010/06/05 06:00
CRDT- 2009/10/29 06:00
PHST- 2009/10/29 06:00 [entrez]
PHST- 2009/10/29 06:00 [pubmed]
PHST- 2010/06/05 06:00 [medline]
AID - 10.1055/s-0029-1186279 [doi]
PST - ppublish
SO  - Planta Med. 2010 Mar;76(5):418-25. doi: 10.1055/s-0029-1186279. Epub 2009 Oct 27.