PMID- 19864244
OWN - NLM
STAT- MEDLINE
DCOM- 20091112
LR  - 20151119
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 132
IP  - 1
DP  - 2009 Jul
TI  - Automated cellular imaging system III for assessing HER2 status in breast cancer 
      specimens: development of a standardized scoring method that correlates with 
      FISH.
PG  - 133-8
LID - 10.1309/AJCPJV0SKAF2PCMY [doi]
AB  - The goal of this study was to assess the performance characteristics of the 
      Automated Cellular Imaging System (ACIS III) for HER2 immunohistochemical 
      analysis. The study was performed on 187 biopsy slides from patients who 
      underwent HER2 testing between January and February 2008. Three scoring methods 
      by the ACIS III were compared with the manual score and fluorescence in situ 
      hybridization (FISH) results for HER2 amplification. The equal distribution score 
      (EQD) method, in which 2 areas each of high-, moderate-, and low-intensity 
      staining were measured, most closely matched the FISH HER2 amplification result. 
      The numbers of immunohistochemically negative (0 or 1+)/FISH+ cases were 
      equivalent for all methods. The EQD method had significantly fewer 2+ cases (n = 
      16) (P < .001) vs the manual method (n = 35) and yielded a higher positive 
      predictive value (38%) for HER2 amplification compared with the manual method 
      (20%). The EQD method may more accurately identify FISH-amplified HER2 cases with 
      fewer 2+ cases that would be "reflexed" to FISH compared with the manual method.
FAU - Minot, Douglas M
AU  - Minot DM
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 
      55905, USA.
FAU - Kipp, Benjamin R
AU  - Kipp BR
FAU - Root, Renee M
AU  - Root RM
FAU - Meyer, Reid G
AU  - Meyer RG
FAU - Reynolds, Carol A
AU  - Reynolds CA
FAU - Nassar, Aziza
AU  - Nassar A
FAU - Henry, Michael R
AU  - Henry MR
FAU - Clayton, Amy C
AU  - Clayton AC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA, Neoplasm)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/metabolism
MH  - Breast Neoplasms/genetics/*metabolism/*pathology
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Gene Amplification
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Image Processing, Computer-Assisted/*methods
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Receptor, ErbB-2/*metabolism
MH  - Reproducibility of Results
MH  - Retrospective Studies
MH  - Young Adult
EDAT- 2009/10/30 06:00
MHDA- 2009/11/13 06:00
CRDT- 2009/10/30 06:00
PHST- 2009/10/30 06:00 [entrez]
PHST- 2009/10/30 06:00 [pubmed]
PHST- 2009/11/13 06:00 [medline]
AID - 132/1/133 [pii]
AID - 10.1309/AJCPJV0SKAF2PCMY [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2009 Jul;132(1):133-8. doi: 10.1309/AJCPJV0SKAF2PCMY.