PMID- 19864871
OWN - NLM
STAT- MEDLINE
DCOM- 20100205
LR  - 20100129
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 126
IP  - 4
DP  - 2009
TI  - Molecular cytogenetic characterization of the human cerebral microvessel 
      endothelial cell line hCMEC/D3.
PG  - 313-7
LID - 10.1159/000253080 [doi]
AB  - The immortalized human cerebral microvessel endothelial cell line hCMEC/D3 has 
      been repeatedly used as a model of human blood-brain barrier (BBB). hCMEC/D3 
      cells between passage 25 and 35 are most often applied in research, remained 
      phenotypically nontransformed, and cells maintained many characteristics of human 
      brain endothelial cells. Also hCMEC/D3 was thought to have conserved a normal 
      diploid karyotype over all these passages. Here we characterized the cell line 
      using high-resolution multicolor fluorescence in situ hybridization (FISH) 
      approaches and revealed a complex karyotype in the 30th passage. Clonal cryptic 
      unbalanced structural rearrangements and numerical aberrations were discovered 
      and described as follows: 45 approximately 48,XX, 
      -X,del(5)(q11)[2],del(9)(q11)[3],+9[3],del(11)(q13 approximately 14)[2], 
      der(14)t(14;21)(q32.33;q22.3)[28],der(15)t(9;15)(p11;p11)[13], 
      dup(15)(p11q11)[5],der(21)t(17;21)(p12;q22)[9],-22[6][cp28]. In summary, a 
      complex karyotype with clonal unbalanced chromosomal rearrangements is present in 
      hCMEC/D3. Thus, we solicit to include molecular cytogenetics in the testing of 
      all cell lines prior to application of their use in complex studies.
CI  - Copyright 2009 S. Karger AG, Basel.
FAU - Mkrtchyan, H
AU  - Mkrtchyan H
AD  - Jena University Hospital, Institute of Human Genetics and Anthropology, Jena, 
      Germany.
FAU - Scheler, S
AU  - Scheler S
FAU - Klein, I
AU  - Klein I
FAU - Fahr, A
AU  - Fahr A
FAU - Couraud, P O
AU  - Couraud PO
FAU - Romero, I A
AU  - Romero IA
FAU - Weksler, B
AU  - Weksler B
FAU - Liehr, T
AU  - Liehr T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091026
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
SB  - IM
MH  - Brain/*blood supply/*cytology
MH  - Cell Line
MH  - Chromosome Aberrations
MH  - Endothelial Cells/*cytology
MH  - Humans
MH  - Karyotyping
MH  - Microvessels/*cytology
EDAT- 2009/10/30 06:00
MHDA- 2010/02/06 06:00
CRDT- 2009/10/30 06:00
PHST- 2009/07/29 00:00 [accepted]
PHST- 2009/10/30 06:00 [entrez]
PHST- 2009/10/30 06:00 [pubmed]
PHST- 2010/02/06 06:00 [medline]
AID - 000253080 [pii]
AID - 10.1159/000253080 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2009;126(4):313-7. doi: 10.1159/000253080. Epub 2009 Oct 
      26.