PMID- 19864893
OWN - NLM
STAT- MEDLINE
DCOM- 20091109
LR  - 20091029
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 125
IP  - 4
DP  - 2009
TI  - Sex chromosome evolution in cotton stainers of the genus Dysdercus (Heteroptera: 
      Pyrrhocoridae).
PG  - 292-305
LID - 10.1159/000235936 [doi]
AB  - The neo-X and neo-Y sex chromosomes of Dysdercus albofasciatus represent a unique 
      model for the study of early stages of sex chromosome evolution since they 
      retained the ability to pair and recombine, in contrast to sex chromosomes in 
      most Heteroptera. Here we examined structure, molecular differentiation, and 
      meiotic behaviour of the D. albofasciatus neo-sex chromosomes. Two related 
      species with the ancestral X0 system, D. chaquensis and D. ruficollis, were used 
      for a comparison. In D. albofasciatus, 2 nucleolar organizer regions (NORs) were 
      identified on the neo-X chromosome using fluorescence in situ hybridization 
      (FISH) with an rDNA probe, whereas a single NOR was found on an autosomal pair in 
      the other 2 species. Genomic in situ hybridization (GISH) differentiated a part 
      of the original X in the neo-X chromosome but not the neo-Y chromosome. The same 
      segment of the neo-X chromosome was identified by Zoo-FISH with a chromosome 
      painting probe derived from the X chromosome of D. ruficollis, indicating that 
      this part is conserved between the species. Immunostaining against the cohesin 
      subunit SMC3 revealed that only terminal regions of the D. albofasciatus 
      neo-Xneo-Y bivalent pair and form a synaptonemal complex, which is in keeping 
      with the occurrence of terminal chiasmata, whereas the interstitial region forms 
      a large loop indicating the absence of homology. These results support the 
      hypothesis that the neo-X chromosome evolved by insertion of the original X 
      chromosome into 1 NOR-bearing autosome in an ancestor carrying the X0 system. As 
      a consequence, the homologue of this NOR-autosome became the neo-Y chromosome. A 
      subsequent inversion followed by transposition of the NOR located on the neo-Y 
      onto the neo-X chromosome resulted in the present neo-sex chromosome system in D. 
      albofasciatus.
CI  - (c) 2009 S. Karger AG, Basel.
FAU - Bressa, M J
AU  - Bressa MJ
AD  - Laboratory of Cytogenetics and Evolution, Faculty of Exact and Natural Sciences, 
      University of Buenos Aires, Buenos Aires, Argentina. mjbressa @ ege.fcen.uba.ar
FAU - Papeschi, A G
AU  - Papeschi AG
FAU - Vitkova, M
AU  - Vitkova M
FAU - Kubickova, S
AU  - Kubickova S
FAU - Fukova, I
AU  - Fukova I
FAU - Pigozzi, M I
AU  - Pigozzi MI
FAU - Marec, F
AU  - Marec F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091027
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Animals
MH  - Biotinylation
MH  - Chromosome Banding
MH  - Chromosome Painting
MH  - Chromosomes
MH  - DNA Probes/chemistry
MH  - DNA, Ribosomal/metabolism
MH  - *Evolution, Molecular
MH  - Female
MH  - Fluorescent Dyes/metabolism
MH  - Heteroptera/*genetics
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Indoles/metabolism
MH  - Karyotyping
MH  - Male
MH  - Meiosis
MH  - Metaphase
MH  - Nucleolus Organizer Region/metabolism
MH  - Species Specificity
MH  - Synaptonemal Complex/metabolism
MH  - *X Chromosome
MH  - *Y Chromosome
EDAT- 2009/10/30 06:00
MHDA- 2009/11/10 06:00
CRDT- 2009/10/30 06:00
PHST- 2009/06/30 00:00 [accepted]
PHST- 2009/10/30 06:00 [entrez]
PHST- 2009/10/30 06:00 [pubmed]
PHST- 2009/11/10 06:00 [medline]
AID - 000235936 [pii]
AID - 10.1159/000235936 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2009;125(4):292-305. doi: 10.1159/000235936. Epub 2009 Oct 
      27.