PMID- 19874375
OWN - NLM
STAT- MEDLINE
DCOM- 20101116
LR  - 20171116
IS  - 1365-2230 (Electronic)
IS  - 0307-6938 (Linking)
VI  - 35
IP  - 4
DP  - 2010 Jun
TI  - Demethylation of promoter regulatory elements contributes to CD70 overexpression 
      in CD4+ T cells from patients with subacute cutaneous lupus erythematosus.
PG  - 425-30
LID - 10.1111/j.1365-2230.2009.03611.x [doi]
AB  - BACKGROUND: Impaired methylation of T-cell DNA is thought to contribute to the 
      development of systemic lupus erythematosus (SLE). CD70 (TNFSF7) is a B-cell 
      costimulatory molecule that contributes to excessive B-cell stimulation in vitro 
      and in vivo. CD70 is overexpressed in CD4+ T cells of patients with SLE, and DNA 
      demethylation occurs in promoter sequences that regulate CD70 expression in SLE 
      CD4+ T cells. However, it is unknown whether the expression and methylation of 
      CD70 in CD4+ T cells are affected in patients with subacute cutaneous lupus 
      erythematosus (SCLE). OBJECTIVE: To compare CD70 expression levels and the 
      methylation status of the CD70 promoter region in CD4+ T cells from patients with 
      SCLE and healthy controls. METHODS: We used real-time RT-PCR to compare messenger 
      RNA levels of CD70 and flow cytometry to compare CD70 protein levels in CD4+ T 
      cells from patients with SCLE and healthy controls. Bisulphite sequencing was 
      used to determine the methylation status of the CD70 promoter region. RESULTS: 
      CD70 is overexpressed at the surface of SCLE CD4+ T cells. Demethylation of the 
      CD70 promoter region was seen in CD4+ T cells from patients with SCLE. 
      CONCLUSIONS: Demethylation of regulatory elements contributes to CD70 
      overexpression in CD4+ T cells of patients with SCLE.
FAU - Luo, Y
AU  - Luo Y
AD  - Department of Dermatology, Epigenetic Research Center, Second Xiangya Hospital, 
      Central South University, Changsha, China.
FAU - Zhao, M
AU  - Zhao M
FAU - Lu, Q
AU  - Lu Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091023
PL  - England
TA  - Clin Exp Dermatol
JT  - Clinical and experimental dermatology
JID - 7606847
RN  - 0 (CD27 Ligand)
RN  - 0 (CD70 protein, human)
SB  - IM
MH  - Adult
MH  - CD27 Ligand/*blood/genetics
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Cells, Cultured
MH  - DNA Methylation
MH  - Female
MH  - Gene Expression Regulation/immunology
MH  - Humans
MH  - Lupus Erythematosus, Cutaneous/genetics/*immunology
MH  - Male
MH  - Middle Aged
MH  - Promoter Regions, Genetic/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Young Adult
EDAT- 2009/10/31 06:00
MHDA- 2010/11/17 06:00
CRDT- 2009/10/31 06:00
PHST- 2009/10/31 06:00 [entrez]
PHST- 2009/10/31 06:00 [pubmed]
PHST- 2010/11/17 06:00 [medline]
AID - CED3611 [pii]
AID - 10.1111/j.1365-2230.2009.03611.x [doi]
PST - ppublish
SO  - Clin Exp Dermatol. 2010 Jun;35(4):425-30. doi: 10.1111/j.1365-2230.2009.03611.x. 
      Epub 2009 Oct 23.