PMID- 19876715
OWN - NLM
STAT- MEDLINE
DCOM- 20100816
LR  - 20100428
IS  - 1438-2199 (Electronic)
IS  - 0939-4451 (Linking)
VI  - 38
IP  - 5
DP  - 2010 May
TI  - Chronic hyperhomocysteinemia impairs vascular function in ovariectomized rat 
      carotid arteries.
PG  - 1515-22
LID - 10.1007/s00726-009-0368-y [doi]
AB  - Homocysteine is an independent risk factor for coronary heart disease, as well as 
      for cerebrovascular and peripheral vascular diseases. The purpose of this study 
      was to investigate the effects of hyperhomocysteinemia (HHcy) on vascular 
      reactivity within carotid artery segments isolated from ovariectomized female 
      rats. Treatment with DL-Hcy thiolactone (1 g/kg body weight per day) reduced the 
      phenylephrine-induced contraction of denuded rings. However, the treatment did 
      not alter KCl-induced contractions, or relaxations induced by sodium 
      nitroprusside or acetylcholine. We report elevated expressions of iNOS, eNOS, and 
      nitrotyrosine in homocysteine-treated rat artery sections. Moreover, the 
      inhibition of NOS by L-NAME, 1,400 W, or L-NNA restored phenylephrine-induced 
      vasoconstriction in carotid artery segments from Hcy-treated rats. In conclusion, 
      our findings show that severe HHCy can promote an acute decrease in the 
      endothelium-independent contractile responses of carotid arteries to adrenergic 
      agonists. This effect was restored by nitric oxide synthase inhibitors, which 
      further supports the involvement of nitric oxide in HHcy-derived vascular 
      dysfunction.
FAU - Celotto, Andrea Carla
AU  - Celotto AC
AD  - Laboratory of Pharmacology, Department of Physics and Chemistry, Faculty of 
      Pharmaceutical Sciences, University of Sao Paulo (USP), Avenida do Cafe 3900, 
      Ribeirao Preto, SP, CEP 14040-903, Brazil. acarla_c@yahoo.com
FAU - Fukada, Sandra Y
AU  - Fukada SY
FAU - Laurindo, Francisco R M
AU  - Laurindo FR
FAU - Haddad, Renato
AU  - Haddad R
FAU - Eberlin, Marcos N
AU  - Eberlin MN
FAU - de Oliveira, Ana Maria
AU  - de Oliveira AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091030
PL  - Austria
TA  - Amino Acids
JT  - Amino acids
JID - 9200312
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
SB  - IM
MH  - Animals
MH  - Carotid Arteries/enzymology/*physiopathology
MH  - Chronic Disease
MH  - Female
MH  - Hyperhomocysteinemia/enzymology/*physiopathology
MH  - Immunohistochemistry
MH  - Nitric Oxide Synthase/metabolism
MH  - *Ovariectomy
MH  - Rats
MH  - Rats, Wistar
EDAT- 2009/10/31 06:00
MHDA- 2010/08/17 06:00
CRDT- 2009/10/31 06:00
PHST- 2009/02/25 00:00 [received]
PHST- 2009/10/10 00:00 [accepted]
PHST- 2009/10/31 06:00 [entrez]
PHST- 2009/10/31 06:00 [pubmed]
PHST- 2010/08/17 06:00 [medline]
AID - 10.1007/s00726-009-0368-y [doi]
PST - ppublish
SO  - Amino Acids. 2010 May;38(5):1515-22. doi: 10.1007/s00726-009-0368-y. Epub 2009 
      Oct 30.