PMID- 19877033
OWN - NLM
STAT- MEDLINE
DCOM- 20100104
LR  - 20220409
IS  - 0004-3591 (Print)
IS  - 0004-3591 (Linking)
VI  - 60
IP  - 11
DP  - 2009 Nov
TI  - Interleukin-6 and type I interferon-regulated genes and chemokines mark disease 
      activity in dermatomyositis.
PG  - 3436-46
LID - 10.1002/art.24936 [doi]
AB  - OBJECTIVE: Up-regulation of whole blood type I interferon (IFN)-driven 
      transcripts and chemokines has been described in a number of autoimmune diseases. 
      An IFN gene expression "signature" is a candidate biomarker in patients with 
      dermatomyositis (DM). This study was performed to evaluate the capacity of 
      IFN-dependent peripheral blood gene and chemokine signatures and levels of 
      proinflammatory cytokines to serve as biomarkers for disease activity in adult 
      and juvenile DM. METHODS: Peripheral blood samples and clinical data were 
      obtained from 56 patients with adult or juvenile DM. The type I IFN gene 
      signature in the whole blood of patients with DM was defined by determining the 
      expression levels of 3 IFN-regulated genes (IFIT1, G1P2, and IRF7) using 
      quantitative real-time reverse transcription-polymerase chain reaction. 
      Multiplexed immunoassays were used to quantify the serum levels of 4 type I 
      IFN-regulated chemokines (IFN-inducible T cell alpha chemoattractant, 
      IFNgamma-inducible 10-kd protein, monocyte chemotactic protein 1 [MCP-1], and 
      MCP-2) and the serum levels of other proinflammatory cytokines, including 
      interleukin-6 (IL-6). RESULTS: DM disease activity correlated significantly with 
      the type I IFN gene signature (r = 0.41, P = 0.007) and with the type I IFN 
      chemokine signature (r = 0.61, P < 0.0001). Furthermore, the serum levels of IL-6 
      were significantly correlated with disease activity (r = 0.45, P = 0.001). In 
      addition, correlations between the serum levels of IL-6 and both the type I IFN 
      gene signature (r = 0.47, P < 0.01) and the type I IFN chemokine signature (r = 
      0.71, P < 0.0001) were detected in patients with DM. CONCLUSION: These results 
      suggest that serum IL-6 production and the type I IFN gene signature are 
      candidate biomarkers for disease activity in adult and juvenile DM. Coregulation 
      of the expression of IFN-driven chemokines and IL-6 suggests a novel pathogenic 
      linkage in DM.
FAU - Bilgic, Hatice
AU  - Bilgic H
AD  - University of Minnesota, Minneapolis, MN 55455, USA.
FAU - Ytterberg, Steven R
AU  - Ytterberg SR
FAU - Amin, Shreyasee
AU  - Amin S
FAU - McNallan, Kelly T
AU  - McNallan KT
FAU - Wilson, Joseph C
AU  - Wilson JC
FAU - Koeuth, Thearith
AU  - Koeuth T
FAU - Ellingson, Sonja
AU  - Ellingson S
FAU - Newman, Brant
AU  - Newman B
FAU - Bauer, Jason W
AU  - Bauer JW
FAU - Peterson, Erik J
AU  - Peterson EJ
FAU - Baechler, Emily C
AU  - Baechler EC
FAU - Reed, Ann M
AU  - Reed AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Biomarkers)
RN  - 0 (CCL2 protein, human)
RN  - 0 (CCL8 protein, human)
RN  - 0 (CXCL10 protein, human)
RN  - 0 (CXCL11 protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL8)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokine CXCL11)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (IFIT1 protein, human)
RN  - 0 (IRF7 protein, human)
RN  - 0 (Interferon Regulatory Factor-7)
RN  - 0 (Interferon Type I)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Ubiquitins)
RN  - 60267-61-0 (ISG15 protein, human)
SB  - IM
CIN - Arthritis Rheum. 2010 Jul;62(7):2181-2; author reply 2182. PMID: 20506372
MH  - Adaptor Proteins, Signal Transducing
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Biomarkers/blood
MH  - Carrier Proteins/blood
MH  - Case-Control Studies
MH  - Chemokine CCL2/blood
MH  - Chemokine CCL8/blood
MH  - Chemokine CXCL10/blood
MH  - Chemokine CXCL11/blood
MH  - Chemokines/*blood
MH  - Child
MH  - Cytokines/blood
MH  - Dermatomyositis/*blood/diagnosis
MH  - Female
MH  - Humans
MH  - Interferon Regulatory Factor-7/blood
MH  - Interferon Type I/*genetics
MH  - Interleukin-6/*blood
MH  - Male
MH  - Middle Aged
MH  - RNA-Binding Proteins
MH  - *Severity of Illness Index
MH  - Ubiquitins/blood
MH  - Young Adult
EDAT- 2009/10/31 06:00
MHDA- 2010/01/05 06:00
CRDT- 2009/10/31 06:00
PHST- 2009/10/31 06:00 [entrez]
PHST- 2009/10/31 06:00 [pubmed]
PHST- 2010/01/05 06:00 [medline]
AID - 10.1002/art.24936 [doi]
PST - ppublish
SO  - Arthritis Rheum. 2009 Nov;60(11):3436-46. doi: 10.1002/art.24936.