PMID- 19877284
OWN - NLM
STAT- MEDLINE
DCOM- 20100812
LR  - 20100517
IS  - 1097-0339 (Electronic)
IS  - 1097-0339 (Linking)
VI  - 38
IP  - 6
DP  - 2010 Jun
TI  - Correlation between morphology and human telomerase gene amplification in 
      bronchial brushing cells for the diagnosis of lung cancer.
PG  - 402-6
LID - 10.1002/dc.21235 [doi]
AB  - The aim of this study was to investigate the frequency of amplification of the 
      human telomerase gene (TERC), as measured by fluorescence in situ hybridization 
      (FISH), in routine liquid-based cytological preparations from bronchial brushing 
      specimens, and to assess the associations between TERC amplification, cytological 
      diagnosis, and cytological morphology, in order to obtain further insight into 
      these associations. Bronchial brushings from 102 patients with lung carcinoma (52 
      squamous-cell carcinomas, 22 adenocarcinomas, 28 small cell lung carcinomas) and 
      40 patients with nonmalignant disease were used. Amplification of TERC was 
      performed using a commercially available two-color FISH probe, and slides were 
      prepared for the SurePath liquid-based Pap test (LPT) using the same samples. 
      Amplification of TERC was significantly associated with histological diagnoses (P 
      < 0.05). Patients with lung cancer, and especially those with nonsmall cell lung 
      cancer, had significantly higher percentages of cells with amplification of TERC 
      than did patients with nonmalignant disease (P < 0.05). Comparing the FISH and 
      LPT results, there was no significant difference in diagnostic sensitivity 
      between the two methods (P > 0.05). However the difference in diagnostic 
      sensitivity of the two methods for squamous-cell carcinoma was significant (P < 
      0.01). FISH can be performed on bronchial brushing specimens to detect 
      amplification of TERC. This test may be an adjunct to cytology screening, 
      especially in squamous-cell carcinoma, and may provide an indication of the 
      potential of individual lesions to progress.
CI  - (c) 2009 Wiley-Liss, Inc.
FAU - Fan, Yi-Bo
AU  - Fan YB
AD  - Department of Pathology, The First Affiliated Hospital and College of Basic 
      Medical Sciences, China Medical University, Shenyang, China.
FAU - Ye, Lin
AU  - Ye L
FAU - Wang, Tian-Yu
AU  - Wang TY
FAU - Wu, Guang-Ping
AU  - Wu GP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bronchoscopy
MH  - Carcinoma, Non-Small-Cell Lung/diagnosis/genetics
MH  - Cytodiagnosis/*methods
MH  - Female
MH  - Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Sensitivity and Specificity
MH  - Small Cell Lung Carcinoma/diagnosis/genetics
MH  - Telomerase/*genetics
EDAT- 2009/10/31 06:00
MHDA- 2010/08/13 06:00
CRDT- 2009/10/31 06:00
PHST- 2009/10/31 06:00 [entrez]
PHST- 2009/10/31 06:00 [pubmed]
PHST- 2010/08/13 06:00 [medline]
AID - 10.1002/dc.21235 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2010 Jun;38(6):402-6. doi: 10.1002/dc.21235.