PMID- 19877522
OWN - NLM
STAT- MEDLINE
DCOM- 20120823
LR  - 20201209
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 38
IP  - 5
DP  - 2009 Sep
TI  - [Improvement of local lymph node assay for cosmetics safety evaluation].
PG  - 585-9
AB  - OBJECTIVE: To improve the local lymph node assay (LLNA) as an alternative method 
      to detect chemicals for both sensitization and irritation. METHODS: The following 
      chemicals: one negative control: 4-Aminobenzoic Acid, three sensitizers: 
      2,4-dinitrochlorobenzene (DNCB), Hexyl cinnamic aldehyde (HCA), 2-Aminophenol 
      (2-APC) and two irritations: potassium hydroxide (KOH), sodium lauryl sulphate 
      (SLS) were selected. According to the normal LLNA, groups of female Balb/c mice 
      were treated with test solutions. The thickness of each ear was measured and each 
      auricle was weighed. On the sixth day, the bilateral draining auricular lymph 
      nodes were excised and weighed. The single cell suspensions were prepared, the 
      lymphocyte were counted and the proliferations of lymph cells were detected by 
      cell counting kit-8 (CCK-8). RESULTS: Significant increase in ear thickness and 
      weight were found in groups of KOH, SLS and DNCB (above 0.5%) (P < 0.05), which 
      could be considered as irritants, whereas irritation were not found in 2-APC and 
      HCA. In the allergic test, three sensitizers showed positive, but different 
      sensitivity were found among each index. HCA, DNCB and 2-APC could all obviously 
      augment the weight of lymph node and the lymphocyte count in different groups (P 
      < 0.05). Conspicuous proliferation of lymphocyte were found in DNCB (all group), 
      HCA (above the middle dose) and 2-APC (high dose) by CCK-8. CONCLUSION: The 
      reformed LLNA using auricle thickness and weighing as observed markers for 
      irritation, and using lymph nodes weighing and proliferation of lymphocyte as 
      observed markers for sensitization, could evaluate both sensitization and 
      irritation at the same time.
FAU - Liu, Zhen
AU  - Liu Z
AD  - Department of Toxicology, the Second Military Medicine University, Shanghai 
      200433, China. liuzhen5_1@163.com
FAU - Liu, Junping
AU  - Liu J
FAU - Wang, Fei
AU  - Wang F
FAU - Xu, Guifeng
AU  - Xu G
FAU - Hou, Juan
AU  - Hou J
FAU - Wan, Xuying
AU  - Wan X
FAU - Zhang, Tianbao
AU  - Zhang T
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Allergens)
RN  - 0 (Cosmetics)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (Hydroxides)
RN  - 0 (Irritants)
RN  - 0 (Potassium Compounds)
RN  - 101-86-0 (hexyl cinnamic aldehyde)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - 7864XYD3JJ (Acrolein)
RN  - WZH3C48M4T (potassium hydroxide)
SB  - IM
MH  - Acrolein/adverse effects/analogs & derivatives
MH  - Allergens/adverse effects/*analysis
MH  - Animals
MH  - *Consumer Product Safety
MH  - Cosmetics/*adverse effects/chemistry
MH  - Dinitrochlorobenzene/adverse effects
MH  - Female
MH  - Hydroxides/adverse effects
MH  - Irritants/adverse effects/*analysis
MH  - *Local Lymph Node Assay
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Potassium Compounds/adverse effects
MH  - Sodium Dodecyl Sulfate/adverse effects
EDAT- 2009/11/03 06:00
MHDA- 2012/08/24 06:00
CRDT- 2009/11/03 06:00
PHST- 2009/11/03 06:00 [entrez]
PHST- 2009/11/03 06:00 [pubmed]
PHST- 2012/08/24 06:00 [medline]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2009 Sep;38(5):585-9.