PMID- 19879932
OWN - NLM
STAT- MEDLINE
DCOM- 20100226
LR  - 20131121
IS  - 1879-3169 (Electronic)
IS  - 0378-4274 (Linking)
VI  - 192
IP  - 2
DP  - 2010 Feb 1
TI  - Comparison of flow cytometry and immunohistochemistry in non-radioisotopic murine 
      lymph node assay using bromodeoxyuridine.
PG  - 229-37
LID - 10.1016/j.toxlet.2009.10.024 [doi]
AB  - Non-radioisotopic local lymph node assay (LLNA) employing 5-bromo-2'-deoxyuridine 
      (BrdU) with flow cytometry (FACS) or immunohistochemistry (IHC) is gaining 
      attention due to a regulatory issue of using radioisotope, (3)H-thymidine, in 
      vivo in traditional LLNA. In this study, to compare the performance of these 
      non-radioisotopic endpoints, 7 chemicals with known sensitizing potencies were 
      examined in LLNA. Mice were topically treated with chemicals or vehicle on both 
      ears for 3 days. After intraperitoneal injection of BrdU, bilateral lymph nodes 
      were isolated separately and undergone respectively, FACS or IHC to determine 
      BrdU incorporated lymph node cells (LNCs). Weight and histology of treated ears 
      were also examined to evaluate chemical-induced edema and irritation. Both FACS 
      and IHC could successively identify the skin sensitizers from non-sensitizers. 
      Comparison of FACS and IHC with traditional LLNA revealed that FACS has a higher 
      sensitivity although both assays produced comparable sensitivity and performance 
      to traditional LLNA. In conclusion, non-radioisotopic LLNA using FACS and IHC can 
      successfully detect sensitizers with a good correlation to traditional LLNA. 
      Notably, FACS showed almost equivalent sensitivity and accuracy to traditional 
      LLNA.
CI  - 2009 Elsevier Ireland Ltd. All rights reserved.
FAU - Jung, Kyoung-Mi
AU  - Jung KM
AD  - Amorepacific Corporation R&D Center, Giheung-gu, Yongin-si, Republic of Korea.
FAU - Bae, Il-Hong
AU  - Bae IH
FAU - Kim, Bae-Hwan
AU  - Kim BH
FAU - Kim, Wang-Ki
AU  - Kim WK
FAU - Chung, Jin-Ho
AU  - Chung JH
FAU - Park, Young-Ho
AU  - Park YH
FAU - Lim, Kyung-Min
AU  - Lim KM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091030
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Bromodeoxyuridine/*analysis/metabolism
MH  - Cell Count
MH  - Ear/pathology
MH  - Female
MH  - Flow Cytometry/*methods
MH  - Immunohistochemistry/*methods
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Organ Size/drug effects
EDAT- 2009/11/03 06:00
MHDA- 2010/02/27 06:00
CRDT- 2009/11/03 06:00
PHST- 2009/09/25 00:00 [received]
PHST- 2009/10/19 00:00 [revised]
PHST- 2009/10/21 00:00 [accepted]
PHST- 2009/11/03 06:00 [entrez]
PHST- 2009/11/03 06:00 [pubmed]
PHST- 2010/02/27 06:00 [medline]
AID - S0378-4274(09)01479-9 [pii]
AID - 10.1016/j.toxlet.2009.10.024 [doi]
PST - ppublish
SO  - Toxicol Lett. 2010 Feb 1;192(2):229-37. doi: 10.1016/j.toxlet.2009.10.024. Epub 
      2009 Oct 30.