PMID- 19882283
OWN - NLM
STAT- MEDLINE
DCOM- 20100201
LR  - 20091102
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 599
DP  - 2010
TI  - Ecophysiological analysis of microorganisms in complex microbial systems by 
      combination of fluorescence in situ hybridization with extracellular staining 
      techniques.
PG  - 117-28
LID - 10.1007/978-1-60761-439-5_8 [doi]
AB  - Ecophysiological analysis and functions of single cells in complex microbial 
      systems can be examined by simple combinations of Fluorescence in situ 
      hybridization (FISH) for identification with various staining techniques 
      targeting functional phenotypes. In this chapter, we describe methods and 
      protocols optimized for the study of extracellular enzymes, surface 
      hydrophobicity and specific surface structures. Although primarily applied to the 
      study of microbes in wastewater treatment (activated sludge and biofilms), the 
      methods may also be used with minor modifications in several other ecosystems.
FAU - Nielsen, Jeppe Lund
AU  - Nielsen JL
AD  - Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg 
      University, Aalborg, Denmark.
FAU - Kragelund, Caroline
AU  - Kragelund C
FAU - Nielsen, Per Halkjaer
AU  - Nielsen PH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Sewage)
SB  - IM
MH  - Biofilms/growth & development
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Sewage/microbiology
MH  - *Water Microbiology
EDAT- 2009/11/03 06:00
MHDA- 2010/02/02 06:00
CRDT- 2009/11/03 06:00
PHST- 2009/11/03 06:00 [entrez]
PHST- 2009/11/03 06:00 [pubmed]
PHST- 2010/02/02 06:00 [medline]
AID - 10.1007/978-1-60761-439-5_8 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;599:117-28. doi: 10.1007/978-1-60761-439-5_8.