PMID- 19884782
OWN - NLM
STAT- MEDLINE
DCOM- 20100201
LR  - 20191210
IS  - 1017-7825 (Print)
IS  - 1017-7825 (Linking)
VI  - 19
IP  - 10
DP  - 2009 Oct
TI  - Use of FT-IR to identify enhanced biomass production and biochemical pool shifts 
      in the marine microalgae, Chlorella ovalis, cultured in media composed of 
      different ratios of deep seawater and fermented animal wastewater.
PG  - 1206-12
AB  - Growth rates, photosystem II photosynthesis, and the levels of chlorophyll a and 
      secondary metabolites of Chlorella ovalis were estimated to determine if they 
      were enhanced by the addition of swine urine (BM) or cow compost water (EP) that 
      had been fermented by soil bacteria to deep seawater (DSW) in an attempt to 
      develop media that enabled batch mass culture at lower costs. Growth of C. ovalis 
      in f/2, f/2-EDTA+BM60%, DSW+BM30%, and DSW+EP60% was enhanced and maintained in 
      the log phase of growth for 16 days. The cell densities of C. ovalis in DSW+EP60% 
      (4.1x10(6) Cells/ml) were higher than those of f/2 (2.9x10(6) Cells/ml), 
      f/2-E+BM60% (3.7x10(6) Cells/ml), and DSW+BM30% (2.7x10(6) Cells/ml). The growth 
      rate was also more favorable for C. ovalis cultured in DSW+EP60% (0.15 day(-1)) 
      than that of C. ovalis cultured in the control medium (f/2) (0.12 day(-1)). 
      Furthermore, the chlorophyll a concentration of C. ovalis cultured in DSW+EP60% 
      (4.56 mg/l) was more than 2-fold greater than that of C. ovalis cultured in f/2 
      (2.35 mg/l). Moreover, the maximal quantum yields of photosystem II at 470 nm 
      (Fv/Fm) were significantly higher in organisms cultured at f/2-E+BM60% (0.53) and 
      DSW+EP60% (0.52) than in the other treatment groups. Finally, Fourier 
      transformation infrared (FT-IR) spectroscopy revealed that C. ovalis grown in 
      DSW+EP60% had more typical peaks and various biochemical pool shifts than those 
      grown in other types of media. Taken together, the results of this study indicate 
      that the use of DSW+EP60% to culture C. ovalis can reduce maintenance expenses 
      and promote higher yields.
FAU - Kim, Mi-Kyung
AU  - Kim MK
AD  - Korea Plankton Culture Collection for Industrialization (KPCCI), Marine Science 
      Research Center, Environmental Research Institute, Yeungnam University, Gyonsan 
      712-749, Korea.
FAU - Jeune, Kyung-Hee
AU  - Jeune KH
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Microbiol Biotechnol
JT  - Journal of microbiology and biotechnology
JID - 9431852
RN  - 0 (Culture Media)
SB  - IM
MH  - Animals
MH  - Bacteria/metabolism
MH  - Biomass
MH  - Biotechnology/economics/*methods
MH  - Cattle
MH  - Chlorella/*chemistry/*growth & development/metabolism
MH  - Culture Media/*chemistry/economics/metabolism
MH  - Fermentation
MH  - Photosynthesis
MH  - Seawater/chemistry
MH  - Spectroscopy, Fourier Transform Infrared/economics/*methods
MH  - Swine
MH  - Waste Disposal, Fluid/*methods
EDAT- 2009/11/04 06:00
MHDA- 2010/02/02 06:00
CRDT- 2009/11/04 06:00
PHST- 2009/11/04 06:00 [entrez]
PHST- 2009/11/04 06:00 [pubmed]
PHST- 2010/02/02 06:00 [medline]
AID - JMB019-10-21 [pii]
PST - ppublish
SO  - J Microbiol Biotechnol. 2009 Oct;19(10):1206-12.