PMID- 19885629
OWN - NLM
STAT- MEDLINE
DCOM- 20100204
LR  - 20151119
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 24
IP  - 6
DP  - 2009 Dec
TI  - Effects of protein kinase Cdelta and phospholipase C-gamma1 on monocyte 
      chemoattractant protein-1 expression in taxol-induced breast cancer cell death.
PG  - 853-8
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a CC chemokine that plays an 
      important role in immune cell migration. It has been reported that chemokines, 
      including MCP-1, are involved in angiogenesis and metastasis. However, the exact 
      role of chemokines in cancer development is still obscure. We investigated the 
      involvement of MCP-1 in taxol-induced breast cancer cell death. The anti-cancer 
      drug taxol induced MCF-7 breast cancer cell death. Treatment with taxol increased 
      the mRNA expression level of MCP-1 in a dose- and time-dependent manner. 
      Up-regulation of MCP-1 by taxol was augmented in cells treated with rottlerin, a 
      specific inhibitor of protein kinase Cdelta (PKCdelta). In addition, 
      taxol-induced MCP-1 expression was reduced by the ectopic expression of PKCdelta 
      in a dose-dependent manner, indicating that PKCdelta plays a negative role in 
      taxol-induced MCP-1 expression in MCF-7 cells. On the other hand, taxol-induced 
      up-regulation of MCP-1 was reduced in cells treated with U73122, an inhibitor of 
      phospholipase C (PLC), and ectopic expression of PLC-gamma1 increased the 
      expression of MCP-1 in taxol-treated MCF-7 cells, indicating that PLC-gamma1 
      functions as a positive regulator in taxol-induced MCP-1 expression. These 
      results indicate that MCP-1 is involved in taxol-induced breast cancer cell death 
      and we propose that taxol induces up-regulation of MCP-1 by affecting both 
      positive and negative regulatory signaling pathways.
FAU - Kim, Yoon Suk
AU  - Kim YS
AD  - Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei 
      University, Wonju 220-710, Korea.
FAU - An, Hyoung Tae
AU  - An HT
FAU - Kim, Jeonghan
AU  - Kim J
FAU - Ko, Jesang
AU  - Ko J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.13 (Protein Kinase C-delta)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
RN  - P88XT4IS4D (Paclitaxel)
SB  - IM
MH  - Breast Neoplasms/drug therapy/enzymology/*metabolism/pathology
MH  - Cell Death/drug effects
MH  - Cell Line, Tumor
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Chemokines/genetics/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Humans
MH  - Paclitaxel/*pharmacology
MH  - Phospholipase C gamma/*metabolism
MH  - Protein Kinase C-delta/*metabolism
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Up-Regulation/drug effects
EDAT- 2009/11/04 06:00
MHDA- 2010/02/05 06:00
CRDT- 2009/11/04 06:00
PHST- 2009/11/04 06:00 [entrez]
PHST- 2009/11/04 06:00 [pubmed]
PHST- 2010/02/05 06:00 [medline]
PST - ppublish
SO  - Int J Mol Med. 2009 Dec;24(6):853-8.