PMID- 19885716
OWN - NLM
STAT- MEDLINE
DCOM- 20100402
LR  - 20231214
IS  - 1880-6562 (Electronic)
IS  - 1880-6546 (Print)
IS  - 1880-6546 (Linking)
VI  - 60
IP  - 1
DP  - 2010 Jan
TI  - Transepithelial calcium transport in prolactin-exposed intestine-like Caco-2 
      monolayer after combinatorial knockdown of TRPV5, TRPV6 and Ca(v)1.3.
PG  - 9-17
LID - 10.1007/s12576-009-0068-0 [doi]
AB  - The milk-producing hormone prolactin (PRL) increases the transcellular intestinal 
      calcium absorption by enhancing apical calcium uptake through voltage-dependent 
      L-type calcium channel (Ca(v)) 1.3. However, the redundancy of apical calcium 
      channels raised the possibility that Ca(v)1.3 may operate with other channels, 
      especially transient receptor potential vanilloid family calcium channels (TRPV) 
      5 or 6, in an interdependent manner. Herein, TRPV5 knockdown (KD), TRPV5/TRPV6, 
      TRPV5/Ca(v)1.3, and TRPV6/Ca(v)1.3 double KD, and TRPV5/TRPV6/Ca(v)1.3 triple KD 
      Caco-2 monolayers were generated by transfecting cells with small interfering 
      RNAs (siRNA). siRNAs downregulated only the target mRNAs, and did not induce 
      compensatory upregulation of the remaining channels. After exposure to 600 ng/mL 
      PRL, the transcellular calcium transport was increased by ~2-fold in scrambled 
      siRNA-treated, TRPV5 KD and TRPV5/TRPV6 KD monolayers, but not in TRPV5/Ca(v)1.3, 
      TRPV6/Ca(v)1.3 and TRPV5/TRPV6/Ca(v)1.3 KD monolayers. The results suggested that 
      Ca(v)1.3 was the sole apical channel responsible for the PRL-stimulated 
      transcellular calcium transport in intestine-like Caco-2 monolayer.
FAU - Nakkrasae, La-iad
AU  - Nakkrasae LI
AD  - Consortium for Calcium and Bone Research (COCAB), Faculty of Science, Mahidol 
      University, Bangkok, Thailand.
FAU - Thongon, Narongrit
AU  - Thongon N
FAU - Thongbunchoo, Jirawan
AU  - Thongbunchoo J
FAU - Krishnamra, Nateetip
AU  - Krishnamra N
FAU - Charoenphandhu, Narattaphol
AU  - Charoenphandhu N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091103
PL  - Japan
TA  - J Physiol Sci
JT  - The journal of physiological sciences : JPS
JID - 101262417
RN  - 0 (CACNA1D protein, human)
RN  - 0 (Calcium Channels)
RN  - 0 (Calcium Channels, L-Type)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (TRPV5 protein, human)
RN  - 0 (TRPV6 protein, human)
RN  - 9002-62-4 (Prolactin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Caco-2 Cells
MH  - Calcium/*metabolism
MH  - Calcium Channels/metabolism/physiology
MH  - Calcium Channels, L-Type/*physiology
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Prolactin/pharmacology
MH  - TRPV Cation Channels/physiology
PMC - PMC10717236
EDAT- 2009/11/04 06:00
MHDA- 2010/04/03 06:00
PMCR- 2009/11/03
CRDT- 2009/11/04 06:00
PHST- 2009/08/13 00:00 [received]
PHST- 2009/10/07 00:00 [accepted]
PHST- 2009/11/04 06:00 [entrez]
PHST- 2009/11/04 06:00 [pubmed]
PHST- 2010/04/03 06:00 [medline]
PHST- 2009/11/03 00:00 [pmc-release]
AID - 68 [pii]
AID - 10.1007/s12576-009-0068-0 [doi]
PST - ppublish
SO  - J Physiol Sci. 2010 Jan;60(1):9-17. doi: 10.1007/s12576-009-0068-0. Epub 2009 Nov 
      3.