PMID- 19886741
OWN - NLM
STAT- MEDLINE
DCOM- 20100603
LR  - 20211203
IS  - 1607-842X (Electronic)
IS  - 0891-6934 (Linking)
VI  - 42
IP  - 8
DP  - 2009
TI  - Silent celiac disease in a cohort of healthy adults.
PG  - 705-8
LID - 10.3109/08916930903214009 [doi]
AB  - BACKGROUND: Recent studies suggest that celiac disease (CD) is common in many 
      developing countries. Because the disease may be under diagnosed in Cuba, we 
      studied the presence of the disease in a group of apparently healthy adult. 
      AIMS/HYPOTHESIS: It was to assess for the first time, the presence of silent CD 
      in a cohort of healthy Cuban adults individuals and to evaluate the tools for 
      diagnosis of CD in this group. METHODS: A total of 200 healthy Cuban adult from 
      Havana City were evaluated. Tissue transglutaminase antibodies (tTGA) were 
      determined by one-step immunochromatographic assay and by commercial ELISA kit. 
      CD specific human leukocyte antigen (HLA) typing was performed by polymerase 
      chain reaction amplification, using sequence-specific primers. In the subject 
      positive for tTGA, the CD was confirmed by intestinal biopsy. RESULTS: From the 
      200 studied individuals, only one subject was identified as positive by both 
      assays, being submitted to duodenal biopsy. Morphological changes consistent with 
      CD were found and also supported by HLA-DQ2 (HLA-DQA1*0501-DQB1*02). In the 
      follow-up after one year, histological recovery was assessed by a second 
      intestinal biopsy and the serological marker became negative. CONCLUSIONS: This 
      study confirms the existence of silent CD among healthy adult in Cuba and 
      highlights the importance of mass screening for this disease among them. The 
      one-step immunochromatographic assay is a good tool for this purpose.
FAU - Galvan, Jose A
AU  - Galvan JA
AD  - Immunodiagnostics and Genomics Division, Center for Genetic Engineering and 
      Biotechnology, Havana, Cuba. armando.galvan@cigb.edu.cu
FAU - Lemos, Gilda
AU  - Lemos G
FAU - Fernandez de Cossio, Maria E
AU  - Fernandez de Cossio ME
FAU - Ruenes, Caridad
AU  - Ruenes C
FAU - Martinez, Yadina
AU  - Martinez Y
FAU - Tejeda, Yelaine
AU  - Tejeda Y
FAU - Roca, Juan
AU  - Roca J
FAU - Palenzuela, Daniel O
AU  - Palenzuela DO
FAU - Novoa, Lidia I
AU  - Novoa LI
FAU - Nazabal, Marcelo
AU  - Nazabal M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Autoimmunity
JT  - Autoimmunity
JID - 8900070
RN  - 0 (Antibodies)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ alpha-Chains)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQA1 antigen)
RN  - 0 (HLA-DQB1 antigen)
RN  - 0 (Immunoglobulin A)
RN  - EC 2.3.2.13 (Protein Glutamine gamma Glutamyltransferase 2)
RN  - EC 2.3.2.13 (Transglutaminases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antibodies/blood/immunology
MH  - Celiac Disease/*diagnosis/diet therapy/*epidemiology/immunology
MH  - Cohort Studies
MH  - Cuba/epidemiology
MH  - Diet, Gluten-Free
MH  - Duodenum/pathology
MH  - Female
MH  - GTP-Binding Proteins/immunology
MH  - HLA-DQ Antigens/immunology
MH  - HLA-DQ alpha-Chains
MH  - HLA-DQ beta-Chains
MH  - *Health
MH  - Humans
MH  - Immunoglobulin A/blood/immunology
MH  - Male
MH  - Middle Aged
MH  - Protein Glutamine gamma Glutamyltransferase 2
MH  - Transglutaminases/immunology
MH  - Young Adult
EDAT- 2009/11/06 06:00
MHDA- 2010/06/04 06:00
CRDT- 2009/11/06 06:00
PHST- 2009/11/06 06:00 [entrez]
PHST- 2009/11/06 06:00 [pubmed]
PHST- 2010/06/04 06:00 [medline]
AID - 10.3109/08916930903214009 [pii]
AID - 10.3109/08916930903214009 [doi]
PST - ppublish
SO  - Autoimmunity. 2009;42(8):705-8. doi: 10.3109/08916930903214009.