PMID- 19886750
OWN - NLM
STAT- MEDLINE
DCOM- 20100415
LR  - 20211203
IS  - 1029-2470 (Electronic)
IS  - 1029-2470 (Linking)
VI  - 44
IP  - 2
DP  - 2010 Feb
TI  - Involvement of mTOR in globular adiponectin-induced generation of reactive oxygen 
      species.
PG  - 128-34
LID - 10.3109/10715760903348328 [doi]
AB  - Globular adiponectin (gAd) induces the generation of reactive oxygen species 
      (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. This 
      study investigated the role of the mammalian target of rapamycin (mTOR) in 
      gAd-induced ROS and NO generation. gAd stimulation induced phosphorylation of 
      mTOR, which peaked at 20 min and dissolved rapidly. Inhibition of 
      phosphatidylinositol 3-kinase activity with wortmannin suppressed gAd-induced 
      phosphorylation of Akt and mTOR. Administration of rapamycin partially reduced 
      gAd-induced generation of intracellular and mitochondrial ROS, but not release of 
      NO. To further confirm the role of mTOR in gAd stimulation, the effect of the 
      activators of AMP-activated protein kinase (AMPK) on gAd-induced mTOR 
      phosphorylation was examined. Pre-treatment with three kinds of AMPK activators, 
      AICAR, 2-deoxy-D-glucose and A-769662, suppressed gAd-induced mTOR 
      phosphorylation. Furthermore, these AMPK activators significantly reduced 
      gAd-evoked intracellular and mitochondrial ROS generation and NO release.
FAU - Fujimoto, Akie
AU  - Fujimoto A
AD  - Department of Preventive Dentistry, Faculty of Dental Science, Kyushu University, 
      Fukuoka, Japan.
FAU - Akifusa, Sumio
AU  - Akifusa S
FAU - Kamio, Noriaki
AU  - Kamio N
FAU - Hirofuji, Takao
AU  - Hirofuji T
FAU - Nonaka, Kazuaki
AU  - Nonaka K
FAU - Yamashita, Yoshihisa
AU  - Yamashita Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Free Radic Res
JT  - Free radical research
JID - 9423872
RN  - 0 (Adiponectin)
RN  - 0 (Androstadienes)
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Pyrones)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Ribonucleotides)
RN  - 0 (Thiophenes)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 360-97-4 (Aminoimidazole Carboxamide)
RN  - 9G2MP84A8W (Deoxyglucose)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - F0X88YW0YK (AICA ribonucleotide)
RN  - P68477CD2C 
      (4-hydroxy-3-(4-(2-hydroxyphenyl)phenyl)-6-oxo-7H-thieno(2,3-b)pyridine-5-carbonitrile)
RN  - W36ZG6FT64 (Sirolimus)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - Adiponectin/antagonists & inhibitors/*pharmacology
MH  - Aminoimidazole Carboxamide/analogs & derivatives/pharmacology
MH  - Androstadienes/pharmacology
MH  - Animals
MH  - Biphenyl Compounds
MH  - Cells, Cultured
MH  - Deoxyglucose/pharmacology
MH  - Intracellular Signaling Peptides and Proteins/*metabolism
MH  - Macrophages/drug effects/metabolism
MH  - Mice
MH  - Nitric Oxide/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Phosphorylation/drug effects
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Pyrones/pharmacology
MH  - Reactive Oxygen Species/*metabolism
MH  - Ribonucleotides/pharmacology
MH  - Sirolimus/pharmacology
MH  - Structure-Activity Relationship
MH  - TOR Serine-Threonine Kinases
MH  - Thiophenes/pharmacology
MH  - Wortmannin
EDAT- 2009/11/06 06:00
MHDA- 2010/04/16 06:00
CRDT- 2009/11/06 06:00
PHST- 2009/11/06 06:00 [entrez]
PHST- 2009/11/06 06:00 [pubmed]
PHST- 2010/04/16 06:00 [medline]
AID - 10.3109/10715760903348328 [pii]
AID - 10.3109/10715760903348328 [doi]
PST - ppublish
SO  - Free Radic Res. 2010 Feb;44(2):128-34. doi: 10.3109/10715760903348328.