PMID- 1988769
OWN - NLM
STAT- MEDLINE
DCOM- 19910228
LR  - 20190725
IS  - 0026-0495 (Print)
IS  - 0026-0495 (Linking)
VI  - 40
IP  - 2
DP  - 1991 Feb
TI  - Influence of portal delivery of insulin on intracellular glucose and lipid 
      metabolism.
PG  - 122-6
AB  - We have investigated whether portal delivery of insulin as a result of 
      intrahepatic islet cell autografts would prevent the development of metabolic 
      alterations. Seven pancreatectomized dogs received islet autografts transplanted 
      into the liver through the portal vein (PD). One year after transplantation, 
      their intravenous glucose tolerance and insulin responses were similar to 
      age-matched control (C) dogs (n = 5). Also, normal triglyceride content in 
      arterial smooth muscle and striated muscle was observed in the dogs with portal 
      insulin delivery in contrast to the substantial increases we observed in 
      pancreatectomized dogs (n = 7) with pancreatic autografts that drained into the 
      systemic circulation (SD). In these dogs, the tissue samples were taken at the 
      age of 3 to 4 years. Triglyceride content (mean +/- SEM) in the aorta was 4.9 +/- 
      1.2 versus 2.6 +/- 0.6 versus 20.7 +/- 8.0 mumol/g (P less than .01) in C, PD, 
      and SD models, respectively. The corresponding values for triglyceride content in 
      striated muscles were 29.1 +/- 1.2, 25.9 +/- 1.5, and 171.4 +/- 46.6 mumol/g (P 
      less than .01). Glucose-6-phosphate dehydrogenase (G-6-PDH) and malic enzyme, key 
      enzymes for lipid synthesis, were also normal in the PD model, in contrast to the 
      fivefold increased activity of these enzymes in the SD model (P less than .01). 
      The glycolytic enzymes, hexokinase (HK) and phosphofructokinase (PFK), were 
      normal compared with the decreased values in the SD. These data indicate that it 
      is possible to normalize glucose and lipid metabolism in arterial walls by portal 
      delivery of insulin, following intrahepatic islet cell transplantation.(ABSTRACT 
      TRUNCATED AT 250 WORDS)
FAU - Falholt, K
AU  - Falholt K
AD  - Novo Research Institute, Copenhagen, Denmark.
FAU - Cutfield, R
AU  - Cutfield R
FAU - Alejandro, R
AU  - Alejandro R
FAU - Volund, A
AU  - Volund A
FAU - Heding, L G
AU  - Heding LG
FAU - Mintz, D H
AU  - Mintz DH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Metabolism
JT  - Metabolism: clinical and experimental
JID - 0375267
RN  - 0 (Insulin)
RN  - 0 (Lipids)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Arteries/enzymology/metabolism
MH  - Dogs
MH  - Fasting
MH  - Glucose/*metabolism
MH  - Glucose Tolerance Test
MH  - Insulin/*pharmacokinetics
MH  - Intracellular Membranes/*metabolism
MH  - Islets of Langerhans Transplantation
MH  - *Lipid Metabolism
MH  - Lipids/blood
MH  - Muscles/enzymology/metabolism
MH  - Pancreatectomy
MH  - Portal System/*metabolism
EDAT- 1991/02/01 00:00
MHDA- 1991/02/01 00:01
CRDT- 1991/02/01 00:00
PHST- 1991/02/01 00:00 [pubmed]
PHST- 1991/02/01 00:01 [medline]
PHST- 1991/02/01 00:00 [entrez]
AID - 0026-0495(91)90161-O [pii]
AID - 10.1016/0026-0495(91)90161-o [doi]
PST - ppublish
SO  - Metabolism. 1991 Feb;40(2):122-6. doi: 10.1016/0026-0495(91)90161-o.