PMID- 19887752
OWN - NLM
STAT- MEDLINE
DCOM- 20091217
LR  - 20190606
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 71
IP  - 10
DP  - 2009 Oct
TI  - Molecular cloning of novel cytochrome P450 1A genes from nine Japanese amphibian 
      species.
PG  - 1407-11
AB  - Novel cytochrome P450 1A (CYP1A) cDNA fragments were isolated from the liver of 
      nine Japanese amphibian species using reverse transcription polymerase chain 
      reaction (RT-PCR). Degenerate PCR primers were used to amplify 122-bp fragments 
      of CYP1A cDNAs. Construction of a phylogenetic tree revealed that urodele and 
      anuran amphibians formed two branches. Within the anuran species, three branches 
      were formed: 1) Ranidae and Rhacophoridae, 2) Bufo japonicus formosus and Hyla 
      japonica, and 3) Xenopus laevis. The cDNA nucleotide sequence of these CYP1A 
      fragments showed identities ranging 72-98% (all), 72-78% (Anura vs. Urodela), 75 
      to 98% (Anura), 81% (Urodela), 74-80% (Xenopus laevis vs. nine Japanese 
      amphibians).
FAU - Nakayama, Shouta M M
AU  - Nakayama SM
AD  - Laboratory of Toxicology, Department of Environmental Veterinary Sciences, 
      Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku N18W9, 
      Sapporo 060-0818, Japan.
FAU - Tanaka-Ueno, Tomoko
AU  - Tanaka-Ueno T
FAU - Sakamoto, Kentaro Q
AU  - Sakamoto KQ
FAU - Fujita, Shoichi
AU  - Fujita S
FAU - Ishizuka, Mayumi
AU  - Ishizuka M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
SB  - IM
MH  - Amphibians/*genetics/*metabolism
MH  - Animals
MH  - *Cloning, Molecular
MH  - Cytochrome P-450 Enzyme System/*genetics/metabolism
MH  - Japan
EDAT- 2009/11/06 06:00
MHDA- 2009/12/18 06:00
CRDT- 2009/11/06 06:00
PHST- 2009/11/06 06:00 [entrez]
PHST- 2009/11/06 06:00 [pubmed]
PHST- 2009/12/18 06:00 [medline]
AID - JST.JSTAGE/jvms/001407 [pii]
AID - 10.1292/jvms.001407 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2009 Oct;71(10):1407-11. doi: 10.1292/jvms.001407.