PMID- 19889049
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20200929
IS  - 1386-6346 (Print)
IS  - 1386-6346 (Linking)
VI  - 39
IP  - 4
DP  - 2009 Apr
TI  - HBcAg-pulsed dendritic cell vaccine induces Th1 polarization and production of 
      hepatitis B virus-specific cytotoxic T lymphocytes.
PG  - 355-65
LID - 10.1111/j.1872-034X.2008.00468.x [doi]
AB  - AIM: Dendritic cells (DCs) pulsed with HBsAg efficiently reverse the immune 
      tolerance to hepatitis B virus (HBV) and induce HBV-specific cytotoxic T 
      lymphocyte (CTL) responses in transgenic mice and healthy volunteers. However, it 
      is not clear whether HBV core antigen (HBcAg)-pulsed DCs can effectively induce 
      CD4(+) helper T cells polarization into Th1, which contribute to the induction 
      and maintenance of HBV-specific CD8(+) T cells in chronic hepatitis B (CHB) 
      patients. To address this issue, we conducted this study and investigated whether 
      HBcAg-pulsed DCs could polarize Th1 cells and induce an HBcAg-specific CTL 
      response. METHODS: HBcAg-pulsed DCs were generated from 21 CHB patients. The 
      capacity of the HBcAg-pulsed DC vaccine to stimulate CD4(+) and CD8(+) T cells to 
      produce IFN-gamma and IL-4 was estimated by intercellular cytokine staining, and 
      the HBcAg-pulsed DCs derived from 10 humam leucocyte antigen (HLA)-A2(+) CHB 
      patients were tested for the induction of HBV-specific CTLs from autologous T 
      cells by pentamer staining. The cytotoxicity of these CTLs was evaluated in vitro 
      by flow cytometry. RESULTS: The HBcAg-pulsed DCs derived from CHB patients 
      exhibited a stronger capacity to stimulate autologous CD4(+) and CD8(+) T cells 
      to release IFN-gamma rather than IL-4, which could induce HBV core 18-27 specific 
      CTLs, suggesting a specific cytotoxicity against T2 cells that had been loaded 
      with the HBV core 18-27 peptide in vitro. CONCLUSION: HBcAg-pulsed DC vaccine 
      derived from CHB patients efficiently induced autologous T cell polarization to 
      Th1 and generation of HBV core 18-27 specific CTLs.
FAU - Chen, Weiwei
AU  - Chen W
AD  - Center for Clinical Laboratory, Bijing Institute of Infectious Diseases, Beijing, 
      China.
FAU - Shi, Ming
AU  - Shi M
FAU - Shi, Feng
AU  - Shi F
FAU - Mao, Yuanli
AU  - Mao Y
FAU - Tang, Zirong
AU  - Tang Z
FAU - Zhang, Bin
AU  - Zhang B
FAU - Zhang, Hui
AU  - Zhang H
FAU - Chen, Liangen
AU  - Chen L
FAU - Chen, Liming
AU  - Chen L
FAU - Xin, Shaojie
AU  - Xin S
FAU - Wang, Fu-Sheng
AU  - Wang FS
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Hepatol Res
JT  - Hepatology research : the official journal of the Japan Society of Hepatology
JID - 9711801
EDAT- 2009/11/06 06:00
MHDA- 2009/11/06 06:01
CRDT- 2009/11/06 06:00
PHST- 2009/11/06 06:00 [entrez]
PHST- 2009/11/06 06:00 [pubmed]
PHST- 2009/11/06 06:01 [medline]
AID - HEP468 [pii]
AID - 10.1111/j.1872-034X.2008.00468.x [doi]
PST - ppublish
SO  - Hepatol Res. 2009 Apr;39(4):355-65. doi: 10.1111/j.1872-034X.2008.00468.x.