PMID- 19889761
OWN - NLM
STAT- MEDLINE
DCOM- 20100112
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 84
IP  - 2
DP  - 2010 Jan
TI  - Adeno-associated virus small rep proteins are modified with at least two types of 
      polyubiquitination.
PG  - 1206-11
LID - 10.1128/JVI.01660-09 [doi]
AB  - Adeno-associated virus (AAV) type 2 and 5 proteins Rep52 and Rep40 were 
      polyubiquitinated during AAV-adenovirus type 5 (Ad5) coinfection and during 
      transient transfection in either the presence or absence of Ad5 E4orf6 and 
      E1b-55k. Polyubiquitination of small Rep proteins via lysine 48 (K48) linkages, 
      normally associated with targeting of proteins for proteasomal degradation, was 
      detected only in the presence of E4orf6. The small Rep proteins were 
      ubiquitinated via lysine 63 (K63) following transfection in either the presence 
      or absence of E4orf6 or following coinfection with Ad5. E4orf6/E1b-55k-dependent 
      K48-specific polyubiquitination of small Rep proteins could be inhibited using 
      small interfering RNA (siRNA) to cullin 5.
FAU - Farris, K David
AU  - Farris KD
AD  - Department of Molecular Microbiology, University of Missouri-Columbia, School of 
      Medicine, Bond Life Sciences Center, Columbia, Missouri 65211, USA.
FAU - Fasina, Olufemi
AU  - Fasina O
FAU - Sukhu, Loretta
AU  - Sukhu L
FAU - Li, Long
AU  - Li L
FAU - Pintel, David J
AU  - Pintel DJ
LA  - eng
GR  - R01 AI046458/AI/NIAID NIH HHS/United States
GR  - R01 AI056310/AI/NIAID NIH HHS/United States
GR  - AI56310/AI/NIAID NIH HHS/United States
GR  - R56 AI046458/AI/NIAID NIH HHS/United States
GR  - AI46458/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20091104
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Adenovirus E4 Proteins)
RN  - 0 (CUL5 protein, human)
RN  - 0 (Cullin Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Ubiquitin)
RN  - 0 (Viral Proteins)
RN  - 137750-19-7 (rep proteins, Adeno-associated virus 2)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 5.99.- (Rep40 protein, adeno-associated virus)
SB  - IM
MH  - Adenoviridae/genetics/metabolism/pathogenicity
MH  - Adenovirus E4 Proteins/genetics/*metabolism
MH  - Cell Line
MH  - Cullin Proteins/genetics/metabolism
MH  - DNA Helicases/*metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Dependovirus/genetics/*metabolism/pathogenicity
MH  - Humans
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Transfection
MH  - Ubiquitin/*metabolism
MH  - Viral Proteins/*metabolism
PMC - PMC2798352
EDAT- 2009/11/06 06:00
MHDA- 2010/01/13 06:00
PMCR- 2010/07/01
CRDT- 2009/11/06 06:00
PHST- 2009/11/06 06:00 [entrez]
PHST- 2009/11/06 06:00 [pubmed]
PHST- 2010/01/13 06:00 [medline]
PHST- 2010/07/01 00:00 [pmc-release]
AID - JVI.01660-09 [pii]
AID - 1660-09 [pii]
AID - 10.1128/JVI.01660-09 [doi]
PST - ppublish
SO  - J Virol. 2010 Jan;84(2):1206-11. doi: 10.1128/JVI.01660-09. Epub 2009 Nov 4.