PMID- 19889779
OWN - NLM
STAT- MEDLINE
DCOM- 20100112
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 84
IP  - 2
DP  - 2010 Jan
TI  - A global analysis of evolutionary conservation among known and predicted 
      gammaherpesvirus microRNAs.
PG  - 716-28
LID - 10.1128/JVI.01302-09 [doi]
AB  - MicroRNAs (miRNAs) are small, noncoding RNAs which posttranscriptionally regulate 
      gene expression. The current release of the miRNA registry lists 16 viruses which 
      encode a total of 146 miRNA hairpins. Strikingly, 139 of these are encoded by 
      members of the herpesvirus family, suggesting an important role for miRNAs in the 
      herpesvirus life cycle. However, with the exception of 7 miRNA hairpins known to 
      be shared by Epstein-Barr virus (EBV) and the closely related rhesus 
      lymphocryptovirus (rLCV), the known herpesvirus miRNAs show little evidence of 
      evolutionary conservation. We have performed a global analysis of miRNA 
      conservation among gammaherpesviruses which is not limited to family members 
      known to encode miRNAs but includes also those which have not been previously 
      analyzed. For this purpose, we have performed a computational prediction of miRNA 
      candidates of all fully sequenced gammaherpesvirus genomes, followed by 
      sequence/structure alignments. Our results indicate that gammaherpesvirus miRNA 
      conservation is limited to two pairs of viral genomes. One is the already-known 
      case of EBV and rLCV. These viruses, however, share significantly more miRNAs 
      than previously thought, as we identified and experimentally verified 10 novel 
      conserved as well as 7 novel nonconserved rLCV pre-miRNA hairpins. The second 
      case consists of rhesus rhadinovirus (RRV), which is predicted to share at least 
      9 pre-miRNAs with the closely related Japanese macaque herpesvirus (JMHV). 
      Although several other gammaherpesviruses are predicted to encode large numbers 
      of clustered miRNAs at conserved genomic loci, no further examples of 
      evolutionarily conserved miRNA sequences were found.
FAU - Walz, Nicole
AU  - Walz N
AD  - Heinrich-Pette-Institute for Experimental Virology and Immunology, Martinistrasse 
      52, D-20251 Hamburg, Germany.
FAU - Christalla, Thomas
AU  - Christalla T
FAU - Tessmer, Uwe
AU  - Tessmer U
FAU - Grundhoff, Adam
AU  - Grundhoff A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091104
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Computational Biology/*methods
MH  - *Conserved Sequence
MH  - *Evolution, Molecular
MH  - Gammaherpesvirinae/classification/*genetics
MH  - Genome, Viral
MH  - Herpesvirus 4, Human/genetics
MH  - Humans
MH  - Lymphocryptovirus/genetics
MH  - Macaca mulatta/virology
MH  - *MicroRNAs/chemistry/genetics/metabolism
MH  - Molecular Sequence Data
MH  - Rhadinovirus/genetics
MH  - Sequence Alignment
PMC - PMC2798347
EDAT- 2009/11/06 06:00
MHDA- 2010/01/13 06:00
PMCR- 2010/07/01
CRDT- 2009/11/06 06:00
PHST- 2009/11/06 06:00 [entrez]
PHST- 2009/11/06 06:00 [pubmed]
PHST- 2010/01/13 06:00 [medline]
PHST- 2010/07/01 00:00 [pmc-release]
AID - JVI.01302-09 [pii]
AID - 1302-09 [pii]
AID - 10.1128/JVI.01302-09 [doi]
PST - ppublish
SO  - J Virol. 2010 Jan;84(2):716-28. doi: 10.1128/JVI.01302-09. Epub 2009 Nov 4.