PMID- 19891998
OWN - NLM
STAT- MEDLINE
DCOM- 20100407
LR  - 20100201
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 71
IP  - 2
DP  - 2010 Feb
TI  - Selection of escape mutation by Pol154-162-specific cytotoxic T cells among 
      chronically HIV-1-infected HLA-B*5401-positive individuals.
PG  - 123-7
LID - 10.1016/j.humimm.2009.10.015 [doi]
AB  - Most escape mutations have been identified on cytotoxic T lymphocyte (CTL) 
      epitopes presented by Caucasian or African human leukocyte antigen (HLA) class I 
      alleles, whereas a limited number of studies have identified the escape mutations 
      on epitopes presented by Asian alleles. HLA-B54 is a common HLA allele in Asian 
      countries. We recently identified five HLA-B*5401-restricted HIV-1-specific CTL 
      epitopes. We here investigated escape mutations in these CTL epitopes in Japanese 
      HIV-1-infected individuals. The frequency of substitution from Glu (E) to Asp (D) 
      at position 7 (FV9-7D) in the Pol 154-162 (FV9) epitope was significantly higher 
      in HLA-B*5401(+) HIV-infected individuals than in HLA-B*5401(-) individuals, 
      whereas substitutions that were significantly higher in HLA-B*5401(+) individuals 
      than in HLA-B*5401(-) individuals were not found in the other four epitopes. 
      FV9-specific CTLs showed reduced killing activity against target cells pulsed 
      with the FV9-7D mutant peptide and failed to kill those infected with the FV9-7D 
      mutant virus, strongly suggesting that FV9-7D is an escape mutant. Furthermore, 
      longitudinal sequence analysis of the FV9 epitope in two HLA-B*5401(+) 
      individuals revealed that the sequence had changed from the wild type to the 
      FV9-7D during the clinical course. Taken together, these results indicate that 
      the FV9-7D escape mutant had been selected by FV9-specific CTLs among chronically 
      HIV-1-infected HLA-B*5401(+) individuals.
CI  - Copyright 2010 American Society for Histocompatibility and Immunogenetics. 
      Published by Elsevier Inc. All rights reserved.
FAU - Hashimoto, Masao
AU  - Hashimoto M
AD  - Division of Viral Immunology, Centers for AIDS Research, Kumamoto University, 
      2-2-1 Honjo, Kumamoto 860-0811, Japan.
FAU - Kitano, Mitsutaka
AU  - Kitano M
FAU - Honda, Kazutaka
AU  - Honda K
FAU - Koizumi, Hirokazu
AU  - Koizumi H
FAU - Dohki, Sachi
AU  - Dohki S
FAU - Oka, Shinichi
AU  - Oka S
FAU - Takiguchi, Masafumi
AU  - Takiguchi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091103
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-B54 antigen)
SB  - IM
MH  - Chronic Disease
MH  - Epitopes, T-Lymphocyte/*genetics/immunology
MH  - HIV Infections/*genetics/*immunology
MH  - HIV-1/genetics/immunology
MH  - HLA-B Antigens/*genetics/immunology
MH  - Humans
MH  - Mutation
MH  - T-Lymphocytes, Cytotoxic/*immunology/virology
EDAT- 2009/11/07 06:00
MHDA- 2010/04/08 06:00
CRDT- 2009/11/07 06:00
PHST- 2009/07/03 00:00 [received]
PHST- 2009/10/06 00:00 [revised]
PHST- 2009/10/27 00:00 [accepted]
PHST- 2009/11/07 06:00 [entrez]
PHST- 2009/11/07 06:00 [pubmed]
PHST- 2010/04/08 06:00 [medline]
AID - S0198-8859(09)00631-4 [pii]
AID - 10.1016/j.humimm.2009.10.015 [doi]
PST - ppublish
SO  - Hum Immunol. 2010 Feb;71(2):123-7. doi: 10.1016/j.humimm.2009.10.015. Epub 2009 
      Nov 3.