PMID- 19892190
OWN - NLM
STAT- MEDLINE
DCOM- 20100106
LR  - 20091106
IS  - 1557-7988 (Electronic)
IS  - 0076-6879 (Linking)
VI  - 463
DP  - 2009
TI  - Isoelectric focusing and two-dimensional gel electrophoresis.
PG  - 515-40
LID - 10.1016/S0076-6879(09)63030-5 [doi]
AB  - By far the highest resolution of all separation techniques for intact proteins in 
      a single analytical run continues to be by the combination of isoelectric 
      focusing (IEF) and SDS-PAGE, originally introduced by O'Farrell [(1975). J. Biol. 
      Chem.250, 4007-4021]. This analytical platform has seen a number of significant 
      advances and applications over the past 25years, including reproducibility using 
      immobilized pH gradient (IPG) strips [Bjellqvist et al. (1982). J. Biochem. 
      Biophys. Methods6, 317-339.], resolution in alkaline IEF using 
      hydroxyethyldisulfide (HED) [Olsson et al. (2002). Proteomics2, 1630-1632], and 
      quantification for differential expression proteomics on intact proteins on a 
      global scale [DIGE; Unlu et al. (1997). Electrophoresis18, 2071-2077]. These 
      major improvements will be highlighted in this chapter alongside the principle 
      and theory of 2D gel electrophoresis, as well as detailed methods for general 2D 
      gel electrophoresis best use protocols.
FAU - Friedman, David B
AU  - Friedman DB
AD  - Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee, 
      USA.
FAU - Hoving, Sjouke
AU  - Hoving S
FAU - Westermeier, Reiner
AU  - Westermeier R
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
RN  - 0 (Indicators and Reagents)
RN  - 0 (Proteins)
SB  - IM
MH  - Animals
MH  - Electrophoresis, Gel, Two-Dimensional/methods
MH  - Electrophoresis, Polyacrylamide Gel/*methods
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Indicators and Reagents/chemistry/supply & distribution
MH  - Isoelectric Focusing/methods
MH  - Models, Biological
MH  - Proteins/analysis/chemistry
RF  - 30
EDAT- 2009/11/07 06:00
MHDA- 2010/01/07 06:00
CRDT- 2009/11/07 06:00
PHST- 2009/11/07 06:00 [entrez]
PHST- 2009/11/07 06:00 [pubmed]
PHST- 2010/01/07 06:00 [medline]
AID - S0076-6879(09)63030-5 [pii]
AID - 10.1016/S0076-6879(09)63030-5 [doi]
PST - ppublish
SO  - Methods Enzymol. 2009;463:515-40. doi: 10.1016/S0076-6879(09)63030-5.