PMID- 19893027
OWN - NLM
STAT- MEDLINE
DCOM- 20100225
LR  - 20231213
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Print)
IS  - 0002-9440 (Linking)
VI  - 175
IP  - 6
DP  - 2009 Dec
TI  - Fluorescence in situ hybridization markers for prediction of cervical lymph node 
      metastases.
PG  - 2637-45
LID - 10.2353/ajpath.2009.090289 [doi]
AB  - The presence of lymph node metastases is associated with poor prognosis in early 
      stage cervical cancer. As of yet, no molecular markers predicting lymph node 
      metastases have been identified. We examined single genetic markers and a 
      composite marker, comprised of three fluorescence in situ hybridization (FISH) 
      probes targeting the genes LAMP3, PROX1, and PRKAA1, in pretreatment cervical 
      biopsies from 16 lymph node positive cases and 15 lymph node negative controls 
      from women with stage IB and IIA cervical cancer. In addition, we determined 
      clonal patterns by including CCND1 to compare the clonal constitution of primary 
      tumors and associated lymph node metastases. The composite FISH marker allowed 
      for classification of patients into those with and without lymph node metastases 
      with a sensitivity and specificity of 75% and 87%, respectively (P = 0.001). The 
      positive predictive value and negative predictive value were 86% and 76%, 
      respectively. Clonal patterns varied among the tumors. In many cases, changes 
      between the primary tumor and lymph node metastases in the most common clones may 
      indicate that certain clones have a growth advantage for establishing metastases 
      in lymph nodes. We conclude that the composite FISH marker may be useful for 
      determining risk for subsequent development of lymph node metastases in patients 
      with cervical cancer.
FAU - Wangsa, Darawalee
AU  - Wangsa D
AD  - Department of Oncology-Pathology, Karolinska Institute, Karolinska University 
      Hospital, Stockholm, Sweden.
FAU - Heselmeyer-Haddad, Kerstin
AU  - Heselmeyer-Haddad K
FAU - Ried, Patricia
AU  - Ried P
FAU - Eriksson, Elina
AU  - Eriksson E
FAU - Schaffer, Alejandro A
AU  - Schaffer AA
FAU - Morrison, Larry E
AU  - Morrison LE
FAU - Luo, Juhua
AU  - Luo J
FAU - Auer, Gert
AU  - Auer G
FAU - Munck-Wikland, Eva
AU  - Munck-Wikland E
FAU - Ried, Thomas
AU  - Ried T
FAU - Lundqvist, Elisabeth Avall
AU  - Lundqvist EA
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20091105
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CCND1 protein, human)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (LAMP3 protein, human)
RN  - 0 (Lysosomal Membrane Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 0 (prospero-related homeobox 1 protein)
RN  - 136601-57-5 (Cyclin D1)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - EC 2.7.11.31 (PRKAA1 protein, human)
SB  - IM
MH  - AMP-Activated Protein Kinases/biosynthesis/genetics
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*genetics
MH  - Cyclin D1/biosynthesis/genetics
MH  - Female
MH  - Homeodomain Proteins/biosynthesis/genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphatic Metastasis/*pathology
MH  - Lysosomal Membrane Proteins/biosynthesis/genetics
MH  - Middle Aged
MH  - Neoplasm Proteins/biosynthesis/genetics
MH  - Neoplasm Staging
MH  - ROC Curve
MH  - Sensitivity and Specificity
MH  - Tumor Suppressor Proteins/biosynthesis/genetics
MH  - Uterine Cervical Neoplasms/*genetics/*pathology
PMC - PMC2789632
EDAT- 2009/11/07 06:00
MHDA- 2010/02/26 06:00
PMCR- 2010/12/01
CRDT- 2009/11/07 06:00
PHST- 2009/11/07 06:00 [entrez]
PHST- 2009/11/07 06:00 [pubmed]
PHST- 2010/02/26 06:00 [medline]
PHST- 2010/12/01 00:00 [pmc-release]
AID - S0002-9440(10)60771-7 [pii]
AID - 10.2353/ajpath.2009.090289 [doi]
PST - ppublish
SO  - Am J Pathol. 2009 Dec;175(6):2637-45. doi: 10.2353/ajpath.2009.090289. Epub 2009 
      Nov 5.