PMID- 19893508
OWN - NLM
STAT- MEDLINE
DCOM- 20100201
LR  - 20211028
IS  - 1750-2799 (Electronic)
IS  - 1754-2189 (Print)
IS  - 1750-2799 (Linking)
VI  - 4
IP  - 12
DP  - 2009
TI  - Array painting: a protocol for the rapid analysis of aberrant chromosomes using 
      DNA microarrays.
PG  - 1722-36
LID - 10.1038/nprot.2009.183 [doi]
AB  - Array painting is a technique that uses microarray technology to rapidly map 
      chromosome translocation breakpoints. Previous methods to map translocation 
      breakpoints have used fluorescence in situ hybridization (FISH) and have 
      consequently been labor-intensive, time-consuming and restricted to the low 
      breakpoint resolution imposed by the use of metaphase chromosomes. Array painting 
      combines the isolation of derivative chromosomes (chromosomes with 
      translocations) and high-resolution microarray analysis to refine the genomic 
      location of translocation breakpoints in a single experiment. In this protocol, 
      we describe array painting by isolation of derivative chromosomes using a MoFlo 
      flow sorter, amplification of these derivatives using whole-genome amplification 
      and hybridization onto commercially available oligonucleotide microarrays. 
      Although the sorting of derivative chromosomes is a specialized procedure 
      requiring sophisticated equipment, the amplification, labeling and hybridization 
      of DNA is straightforward, robust and can be completed within 1 week. The 
      protocol described produces good quality data; however, array painting is equally 
      achievable using any combination of the available alternative methodologies for 
      chromosome isolation, amplification and hybridization.
FAU - Gribble, Susan M
AU  - Gribble SM
AD  - Human Genetics, Sulston Laboratories, The Wellcome Trust Sanger Institute, 
      Wellcome Trust Genome Campus, Cambridge, UK. smg@sanger.ac.uk
FAU - Ng, Bee Ling
AU  - Ng BL
FAU - Prigmore, Elena
AU  - Prigmore E
FAU - Fitzgerald, Tomas
AU  - Fitzgerald T
FAU - Carter, Nigel P
AU  - Carter NP
LA  - eng
GR  - 077008/WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091105
PL  - England
TA  - Nat Protoc
JT  - Nature protocols
JID - 101284307
SB  - IM
MH  - Cell Culture Techniques
MH  - Cell Line
MH  - Chromosome Mapping/*methods
MH  - Flow Cytometry
MH  - Gene Library
MH  - Humans
MH  - Nucleic Acid Amplification Techniques
MH  - Oligonucleotide Array Sequence Analysis/methods
MH  - *Translocation, Genetic
PMC - PMC3330750
MID - UKMS30439
OID - NLM: UKMS30439
EDAT- 2009/11/07 06:00
MHDA- 2010/02/02 06:00
PMCR- 2012/04/20
CRDT- 2009/11/07 06:00
PHST- 2009/11/07 06:00 [entrez]
PHST- 2009/11/07 06:00 [pubmed]
PHST- 2010/02/02 06:00 [medline]
PHST- 2012/04/20 00:00 [pmc-release]
AID - nprot.2009.183 [pii]
AID - 10.1038/nprot.2009.183 [doi]
PST - ppublish
SO  - Nat Protoc. 2009;4(12):1722-36. doi: 10.1038/nprot.2009.183. Epub 2009 Nov 5.