PMID- 19894108
OWN - NLM
STAT- MEDLINE
DCOM- 20100408
LR  - 20220409
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 26
IP  - 11-12
DP  - 2009 Nov-Dec
TI  - Cytogenetic analysis of human embryos and embryonic stem cells derived from 
      monopronuclear zygotes.
PG  - 583-9
LID - 10.1007/s10815-009-9355-1 [doi]
AB  - PURPOSE: By comparing the chromosomal constitution among the arrested 
      cleavage-stage embryos, blastocysts and human embryonic stem cells (hESCs) which 
      are all derived from monopronuclear (1PN) zygotes, it is aimed to determine 
      whether chromosomally normal embryos can be reliably selected by blastocyst 
      culture. METHODS: After 1PN zygotes are sequentially cultured for 5 days, the 
      blastocysts and arrested cleavage-stage embryos were analyzed by fluorescence in 
      situ hybridization (FISH) with probes for chromosomes 18, X and Y; G-banding 
      analysis was adopted to analyze the karyotype of 1PN hESCs. RESULTS: The diploid 
      rate of blastocysts was 74.6%, which was significantly (P < 0.001) higher than 
      that of arrested cleavage-stage embryos (31.6%), and the diploid rate of hESCs 
      was 97.0%, which was significantly (P < 0.01) higher than that of blastocysts; 
      the haploid embryos were excluded by blastocyst culture; nevertheless, there 
      still existed such chromosomal abnormalities as mosaic and monosomic in 
      blastocysts and trisomy in hESCs. CONCLUSIONS: Blastocyst culture is an effective 
      method to select against chromosomal abnormalities, especially the haploids in 
      1PN embryos; however, development to the blastocyst stage is not a reliable 
      marker for mosaicism or aneuploidy.
FAU - Liao, Hongqing
AU  - Liao H
AD  - Institute of Reproductive and Stem Cell Engineering, Central South University, 
      Changsha, Hunan, China.
FAU - Zhang, Shuoping
AU  - Zhang S
FAU - Cheng, Dehua
AU  - Cheng D
FAU - Ouyang, Qi
AU  - Ouyang Q
FAU - Lin, Ge
AU  - Lin G
FAU - Gu, Yifan
AU  - Gu Y
FAU - Lu, Changfu
AU  - Lu C
FAU - Gong, Fei
AU  - Gong F
FAU - Lu, Guangxiu
AU  - Lu G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091106
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
SB  - IM
MH  - Blastocyst/cytology/*ultrastructure
MH  - Chi-Square Distribution
MH  - Chromosome Aberrations/*embryology
MH  - *Chromosomes, Human
MH  - Cytogenetic Analysis/*methods
MH  - Embryonic Stem Cells/cytology/*ultrastructure
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Ploidies
MH  - Pregnancy
MH  - Preimplantation Diagnosis/methods
PMC - PMC2799555
EDAT- 2009/11/07 06:00
MHDA- 2010/04/09 06:00
PMCR- 2010/12/01
CRDT- 2009/11/07 06:00
PHST- 2009/07/06 00:00 [received]
PHST- 2009/10/08 00:00 [accepted]
PHST- 2009/11/07 06:00 [entrez]
PHST- 2009/11/07 06:00 [pubmed]
PHST- 2010/04/09 06:00 [medline]
PHST- 2010/12/01 00:00 [pmc-release]
AID - 9355 [pii]
AID - 10.1007/s10815-009-9355-1 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2009 Nov-Dec;26(11-12):583-9. doi: 
      10.1007/s10815-009-9355-1. Epub 2009 Nov 6.