PMID- 19894228
OWN - NLM
STAT- MEDLINE
DCOM- 20100225
LR  - 20151119
IS  - 1521-3765 (Electronic)
IS  - 0947-6539 (Linking)
VI  - 16
IP  - 2
DP  - 2010 Jan 11
TI  - Preparation and cellular uptake of pH-dependent fluorescent single-wall carbon 
      nanotubes.
PG  - 556-61
LID - 10.1002/chem.200901168 [doi]
AB  - Fluorescent single-wall carbon nanotubes (SWCNTs) were prepared by mixing cut 
      SWCNTs with acridine orange (AO). The optical absorbance and fluorescence 
      characteristics of AO-SWCNT conjugates display interesting pH-dependent 
      properties. Fluorescence microscopy in combination with transmission electron 
      microscopy proves that AO-SWCNTs can enter HeLa cells and are located inside 
      lysosomes. The endocytosis-inhibiting tests show that the clathrin-mediated 
      endocytosis is a key step in the internalization process. The internalized 
      AO-SWCNTs remain inside lysosomes for more than a week and have little effect on 
      cell proliferation. These findings may be useful in understanding the SWCNT-based 
      intracellular drug delivery mechanism and help to develop new intracellular drug 
      transporters.
FAU - Zhang, Xiaoke
AU  - Zhang X
AD  - School of Chemistry and Chemical Technology, Shanghai Jiao Tong University, 
      Shanghai 200240, China.
FAU - Meng, Lingjie
AU  - Meng L
FAU - Wang, Xuefeng
AU  - Wang X
FAU - Lu, Qinghua
AU  - Lu Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Chemistry
JT  - Chemistry (Weinheim an der Bergstrasse, Germany)
JID - 9513783
RN  - 0 (Nanotubes, Carbon)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Cell Proliferation/drug effects
MH  - Drug Delivery Systems
MH  - Endocytosis
MH  - HeLa Cells
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Lysosomes/*metabolism
MH  - Microscopy, Electron, Transmission
MH  - Microscopy, Fluorescence
MH  - *Nanotubes, Carbon
EDAT- 2009/11/07 06:00
MHDA- 2010/02/26 06:00
CRDT- 2009/11/07 06:00
PHST- 2009/11/07 06:00 [entrez]
PHST- 2009/11/07 06:00 [pubmed]
PHST- 2010/02/26 06:00 [medline]
AID - 10.1002/chem.200901168 [doi]
PST - ppublish
SO  - Chemistry. 2010 Jan 11;16(2):556-61. doi: 10.1002/chem.200901168.