PMID- 19897195
OWN - NLM
STAT- MEDLINE
DCOM- 20100614
LR  - 20131121
IS  - 1879-1484 (Electronic)
IS  - 0021-9150 (Linking)
VI  - 209
IP  - 2
DP  - 2010 Apr
TI  - Dendritic cells pulsed with malondialdehyde modified low density lipoprotein 
      aggravate atherosclerosis in Apoe(-/-) mice.
PG  - 436-41
LID - 10.1016/j.atherosclerosis.2009.10.003 [doi]
AB  - OBJECTIVE: Atherosclerosis is an inflammatory disease involving activation of 
      adaptive and innate immune responses to modified lipoproteins. Dendritic cells 
      (DCs), which are professional antigen-presenting cells that activate T cells, are 
      present in atherosclerotic lesions but their role for atherosclerosis-related 
      immunity is unclear. METHODS AND RESULTS: To evaluate the role of DC in 
      atherosclerosis, DCs pulsed with malondialdehyde modified low density lipoprotein 
      (MDA-LDL) were transferred into Apoe(-/-) mice. The extent of disease was 
      measured in the aortic root and was compared to that in animals treated with 
      Keyhole Limpet Hemocyanin (KLH) pulsed DCs and to untreated animals. Mice 
      receiving MDA-LDL pulsed DCs showed significantly larger atherosclerotic lesions 
      compared to controls, with increased inflammation in the lesions and 
      antigen-specific immune responses. Furthermore, MDA-LDL administration in 
      complete Freund's adjuvant, which is atheroprotective, led to the induction of 
      regulatory T cells whereas MDA-LDL-DCs treatment did not, suggesting that 
      modulation of immune properties can result in different effects on 
      atherosclerosis. CONCLUSIONS: DCs presenting components of LDL promote specific 
      immunity to their antigen, increase lesion inflammation and accelerate 
      atherosclerosis.
CI  - Copyright 2009 Elsevier Ireland Ltd. All rights reserved.
FAU - Hjerpe, Charlotta
AU  - Hjerpe C
AD  - Centre for Molecular Medicine and Department of Medicine, Karolinska Institutet, 
      Stockholm, Sweden.
FAU - Johansson, Daniel
AU  - Johansson D
FAU - Hermansson, Andreas
AU  - Hermansson A
FAU - Hansson, Goran K
AU  - Hansson GK
FAU - Zhou, Xinghua
AU  - Zhou X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091107
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
RN  - 0 (Apolipoproteins E)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (malondialdehyde-low density lipoprotein, mouse)
RN  - 4Y8F71G49Q (Malondialdehyde)
SB  - IM
MH  - Animals
MH  - Apolipoproteins E/*deficiency
MH  - Atherosclerosis/immunology/*pathology
MH  - Dendritic Cells/immunology/*pathology
MH  - Female
MH  - Lipoproteins, LDL/*pharmacology
MH  - Malondialdehyde/*analogs & derivatives/pharmacology
MH  - Mice
MH  - T-Lymphocytes, Regulatory/immunology
EDAT- 2009/11/10 06:00
MHDA- 2010/06/15 06:00
CRDT- 2009/11/10 06:00
PHST- 2009/05/20 00:00 [received]
PHST- 2009/09/28 00:00 [revised]
PHST- 2009/10/02 00:00 [accepted]
PHST- 2009/11/10 06:00 [entrez]
PHST- 2009/11/10 06:00 [pubmed]
PHST- 2010/06/15 06:00 [medline]
AID - S0021-9150(09)00830-2 [pii]
AID - 10.1016/j.atherosclerosis.2009.10.003 [doi]
PST - ppublish
SO  - Atherosclerosis. 2010 Apr;209(2):436-41. doi: 
      10.1016/j.atherosclerosis.2009.10.003. Epub 2009 Nov 7.