PMID- 19898485
OWN - NLM
STAT- MEDLINE
DCOM- 20091215
LR  - 20230425
IS  - 1548-7105 (Electronic)
IS  - 1548-7091 (Print)
IS  - 1548-7091 (Linking)
VI  - 6
IP  - 12
DP  - 2009 Dec
TI  - Imaging neural activity in worms, flies and mice with improved GCaMP calcium 
      indicators.
PG  - 875-81
LID - 10.1038/nmeth.1398 [doi]
AB  - Genetically encoded calcium indicators (GECIs) can be used to image activity in 
      defined neuronal populations. However, current GECIs produce inferior signals 
      compared to synthetic indicators and recording electrodes, precluding detection 
      of low firing rates. We developed a single-wavelength GCaMP2-based GECI (GCaMP3), 
      with increased baseline fluorescence (3-fold), increased dynamic range (3-fold) 
      and higher affinity for calcium (1.3-fold). We detected GCaMP3 fluorescence 
      changes triggered by single action potentials in pyramidal cell dendrites, with 
      signal-to-noise ratio and photostability substantially better than those of 
      GCaMP2, D3cpVenus and TN-XXL. In Caenorhabditis elegans chemosensory neurons and 
      the Drosophila melanogaster antennal lobe, sensory stimulation-evoked 
      fluorescence responses were significantly enhanced with GCaMP3 (4-6-fold). In 
      somatosensory and motor cortical neurons in the intact mouse, GCaMP3 detected 
      calcium transients with amplitudes linearly dependent on action potential number. 
      Long-term imaging in the motor cortex of behaving mice revealed large 
      fluorescence changes in imaged neurons over months.
FAU - Tian, Lin
AU  - Tian L
AD  - Howard Hughes Medical Institute, Ashburn, Virginia, USA.
FAU - Hires, S Andrew
AU  - Hires SA
FAU - Mao, Tianyi
AU  - Mao T
FAU - Huber, Daniel
AU  - Huber D
FAU - Chiappe, M Eugenia
AU  - Chiappe ME
FAU - Chalasani, Sreekanth H
AU  - Chalasani SH
FAU - Petreanu, Leopoldo
AU  - Petreanu L
FAU - Akerboom, Jasper
AU  - Akerboom J
FAU - McKinney, Sean A
AU  - McKinney SA
FAU - Schreiter, Eric R
AU  - Schreiter ER
FAU - Bargmann, Cornelia I
AU  - Bargmann CI
FAU - Jayaraman, Vivek
AU  - Jayaraman V
FAU - Svoboda, Karel
AU  - Svoboda K
FAU - Looger, Loren L
AU  - Looger LL
LA  - eng
GR  - HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
DEP - 20091108
PL  - United States
TA  - Nat Methods
JT  - Nature methods
JID - 101215604
RN  - SY7Q814VUP (Calcium)
SB  - IM
CIN - Nat Methods. 2009 Dec;6(12):871-2. PMID: 19935839
MH  - Animals
MH  - Brain/metabolism
MH  - Caenorhabditis elegans/*cytology/metabolism
MH  - Calcium/*metabolism
MH  - Cell Line
MH  - Drosophila melanogaster/*cytology/metabolism
MH  - Fluorescence Resonance Energy Transfer
MH  - Humans
MH  - Mice
MH  - Neurons/*metabolism
PMC - PMC2858873
MID - NIHMS186333
OID - NLM: HHMIMS186333
COIS- Competing interests statement The authors declare no competing financial 
      interests.
EDAT- 2009/11/10 06:00
MHDA- 2009/12/16 06:00
PMCR- 2010/06/01
CRDT- 2009/11/10 06:00
PHST- 2009/03/02 00:00 [received]
PHST- 2009/10/09 00:00 [accepted]
PHST- 2009/11/10 06:00 [entrez]
PHST- 2009/11/10 06:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
PHST- 2010/06/01 00:00 [pmc-release]
AID - nmeth.1398 [pii]
AID - 10.1038/nmeth.1398 [doi]
PST - ppublish
SO  - Nat Methods. 2009 Dec;6(12):875-81. doi: 10.1038/nmeth.1398. Epub 2009 Nov 8.