PMID- 19899971
OWN - NLM
STAT- MEDLINE
DCOM- 20091221
LR  - 20190902
IS  - 1460-2202 (Electronic)
IS  - 0271-3683 (Linking)
VI  - 34
IP  - 7
DP  - 2009 Jul
TI  - TrkB-T1 receptors on Muller cells play critical role in brain-derived 
      neurotrophic factor-mediated photoreceptor protection against phototoxicity.
PG  - 580-8
AB  - PURPOSE: To determine how brain-derived neurotrophic factor (BDNF) protects 
      photoreceptors against phototoxicity. METHODS: Iris pigment epithelial cells 
      (IPE) that were transduced with different concentrations of adeno-associated 
      virus (AAV) mediated BDNF (AAV-BDNF-IPE) were transplanted into the subretinal 
      space of rats. We also injected small interfering RNAs (siRNAs) for TrkB, a BDNF 
      receptor. The rats were exposed to continuous light to induce phototoxicity. We 
      examined the expression of TrkB in the retina by Western blot and 
      immunohistochemistry. RESULTS: Significant photoreceptor protection was detected 
      when more than 1 x 10(7) capsids/ml AAV-BDNF was transplanted. An intravitreal 
      injection of siRNAs showed that the photoreceptor protection by AAV-BDNF-IPE was 
      reduced by injecting the siRNA of TrkB-T1, one of the TrkB isoforms. TrkB-T1 was 
      slightly upregulated by Western blot, and one of the cells that upregulated 
      TrkB-T1 was Muller cells by immunohistochemistry. CONCLUSION: We conclude that 
      Muller cells are one of the cells responsible for the expression of TrkBs, and 
      TrkB-T1 may play a role in the protection of photoreceptors against 
      phototoxicity.
FAU - Saito, Takae
AU  - Saito T
AD  - Division of Clinical Cell Therapy, Center for Translational and Advanced Animal 
      Research, Miyagi, Japan.
FAU - Abe, Toshiaki
AU  - Abe T
FAU - Wakusawa, Ryosuke
AU  - Wakusawa R
FAU - Sato, Hajime
AU  - Sato H
FAU - Asai, Harunobu
AU  - Asai H
FAU - Tokita-Ishikawa, Yumi
AU  - Tokita-Ishikawa Y
FAU - Nishida, Kohji
AU  - Nishida K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*physiology
MH  - Cell Transplantation
MH  - Dependovirus/genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Genetic Vectors
MH  - Immunohistochemistry
MH  - Iris/cytology
MH  - Light/adverse effects
MH  - Neuroglia/*metabolism
MH  - Photoreceptor Cells, Vertebrate/*radiation effects
MH  - Pigment Epithelium of Eye/metabolism/transplantation
MH  - RNA, Small Interfering/physiology
MH  - Radiation Injuries, Experimental/etiology/*prevention & control
MH  - Rats
MH  - Rats, Long-Evans
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/*physiology
MH  - Retinal Degeneration/etiology/*prevention & control
MH  - Transfection
EDAT- 2009/11/11 06:00
MHDA- 2009/12/22 06:00
CRDT- 2009/11/11 06:00
PHST- 2009/11/11 06:00 [entrez]
PHST- 2009/11/11 06:00 [pubmed]
PHST- 2009/12/22 06:00 [medline]
AID - 10.1080/02713680902972358 [pii]
AID - 10.1080/02713680902972358 [doi]
PST - ppublish
SO  - Curr Eye Res. 2009 Jul;34(7):580-8. doi: 10.1080/02713680902972358.