PMID- 19901160
OWN - NLM
STAT- MEDLINE
DCOM- 20100121
LR  - 20160726
IS  - 1524-4563 (Electronic)
IS  - 0194-911X (Linking)
VI  - 55
IP  - 1
DP  - 2010 Jan
TI  - Induction of mitogen-activated protein kinases is proportional to the amount of 
      pressure overload.
PG  - 137-43
LID - 10.1161/HYPERTENSIONAHA.109.135467 [doi]
AB  - Pressure overload has been shown to induce mitogen activated protein kinases 
      (MAPKs) and reactivate the atrial natriuretic factor in the heart. To test the 
      sensitivity of these signals to pressure overload, we assayed the activity of 
      MAPKs extracellular signal-regulated kinase, c-Jun N-terminal kinase 1, and p38 
      in protein lysates from the left ventricle (LV) or white blood cells (WBC) 
      isolated from aortic banded mice with varying levels of pressure overload. In 
      separated mice we measured atrial natriuretic factor mRNA levels by Northern 
      blotting. As expected, a significant induction of atrial natriuretic factor mRNA 
      levels was observed after aortic banding, and it significantly correlated with 
      the trans-stenotic systolic pressure gradient but not with the LV weight:body 
      weight ratio. In contrast, a significant correlation with systolic pressure 
      gradient or LV weight:body weight ratio was observed for all of the MAPK activity 
      detected in LV samples or WBCs. Importantly, LV activation of MAPKs significantly 
      correlated with their activation in WBCs from the same animal. To test whether 
      MAPK activation in WBCs might reflect uncontrolled blood pressure levels in 
      humans, we assayed extracellular signal-regulated kinase, c-Jun N-terminal kinase 
      1, and p38 activation in WBCs isolated from normotensive volunteers, hypertensive 
      patients with controlled blood pressure values, or hypertensive patients with 
      uncontrolled blood pressure values. Interestingly, in hypertensive patients with 
      controlled blood pressure values, LV mass and extracellular signal-regulated 
      kinase phosphorylation were significantly reduced compared with those in 
      hypertensive patients with uncontrolled blood pressure values. These results 
      suggest that MAPKs are sensors of pressure overload and that extracellular 
      signal-regulated kinase activation in WBCs might be used as a novel surrogate 
      biomarker of uncontrolled human hypertension.
FAU - Esposito, Giovanni
AU  - Esposito G
AD  - Division of Cardiology, Federico II University, Via Pansini 5, 80131 Naples, 
      Italy. espogiov@unina.it
FAU - Perrino, Cinzia
AU  - Perrino C
FAU - Schiattarella, Gabriele Giacomo
AU  - Schiattarella GG
FAU - Belardo, Lorena
AU  - Belardo L
FAU - di Pietro, Elisa
AU  - di Pietro E
FAU - Franzone, Anna
AU  - Franzone A
FAU - Capretti, Giuliana
AU  - Capretti G
FAU - Gargiulo, Giuseppe
AU  - Gargiulo G
FAU - Pironti, Gianluigi
AU  - Pironti G
FAU - Cannavo, Alessandro
AU  - Cannavo A
FAU - Sannino, Anna
AU  - Sannino A
FAU - Izzo, Raffaele
AU  - Izzo R
FAU - Chiariello, Massimo
AU  - Chiariello M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091109
PL  - United States
TA  - Hypertension
JT  - Hypertension (Dallas, Tex. : 1979)
JID - 7906255
RN  - 85637-73-6 (Atrial Natriuretic Factor)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 8)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
CIN - Hypertension. 2010 Jan;55(1):23-5. PMID: 19901156
MH  - Animals
MH  - Aorta/physiopathology
MH  - Atrial Natriuretic Factor/genetics
MH  - Blood Pressure/*physiology
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Constriction, Pathologic
MH  - Enzyme Activation
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Hypertension/enzymology/genetics/*physiopathology
MH  - Hypertrophy
MH  - Leukocytes/enzymology
MH  - MAP Kinase Signaling System/*physiology
MH  - Male
MH  - Mice
MH  - Middle Aged
MH  - Mitogen-Activated Protein Kinase 1/blood/metabolism
MH  - Mitogen-Activated Protein Kinase 8/blood/metabolism
MH  - Mitogen-Activated Protein Kinases/blood/*metabolism
MH  - Myocardium/metabolism/pathology
MH  - Phosphorylation
MH  - Pressure
MH  - p38 Mitogen-Activated Protein Kinases/blood/metabolism
EDAT- 2009/11/11 06:00
MHDA- 2010/01/22 06:00
CRDT- 2009/11/11 06:00
PHST- 2009/11/11 06:00 [entrez]
PHST- 2009/11/11 06:00 [pubmed]
PHST- 2010/01/22 06:00 [medline]
AID - HYPERTENSIONAHA.109.135467 [pii]
AID - 10.1161/HYPERTENSIONAHA.109.135467 [doi]
PST - ppublish
SO  - Hypertension. 2010 Jan;55(1):137-43. doi: 10.1161/HYPERTENSIONAHA.109.135467. 
      Epub 2009 Nov 9.